CoraLite® Plus 647 Anti-Rat CD11b/c (OX42)

CD11b/c Monoclonal Antibody for FC
Cat No. CL647-65229
Clone No.OX42

Host / Isotype

Mouse / IgG2a, kappa

Reactivity

rat and More (1)

Applications

IF, FC

CD11b, integrin alpha M, Itgam, OX42

Formulation:  PBS and Azide
PBS and Azide
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive FC detected inWistar rat splenocytes

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.5 ug per 10^6 cells in 100 μl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

CL647-65229 targets CD11b/c in IF, FC applications and shows reactivity with rat samples.

Tested Reactivity rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG2a, kappa
Class Monoclonal
Type Antibody
Immunogen

fusion protein

Predict reactive species
Full Name integrin alpha M
Calculated Molecular Weight127 kDa
GenBank Accession NumberNM_012711
Gene Symbol CD11b
Gene ID (NCBI) 25021
RRIDAB_2935018
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
FormLiquid
Purification MethodAffinity purification
UNIPROT IDQ9JI30
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b (integrin alpha M or CR3A) and CD11c (integrin alpha X) are members of the integrin alpha chain family and can form α/β heterodimers with CD18 (integrin β2). CD11b/CD18 is a receptor for the complement protein fragment C3bi, and is also a receptor for fibrinogen, factor X, and ICAM1 (PMID: 2971974; 15485828). CD11c/CD18 acts as a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis (PMID: 1671533). The OX-42 antibody recognizes a common epitope shared between CD11b and CD11c. It binds to C3bi receptor and is known to block C3bi binding (PMID: 3512425; 8449218).

Protocols

Product Specific Protocols
FC protocol for CL Plus 647 CD11b/c antibody CL647-65229Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Cell Biosci

Tie2-expressing monocytes/macrophages promote angiogenesis in chronically ischaemic brain tissue

Authors - Chuyang Tai
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