Anti-Human CD14 (UCHM-1)

CD14 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

UCHM-1

Cat no : 65056-1-Ig

Synonyms

CD14, CD14 molecule



Tested Applications

Positive FC detected inhuman peripheral blood monocytes

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

65056-1-Ig targets CD14 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Thymocytes and peripheral blood lymphocytes from a remission AML patient
Full Name CD14 molecule
Calculated molecular weight 375 aa, 40 kDa
GenBank accession numberBC010507
Gene symbol CD14
Gene ID (NCBI) 929
Conjugate Unconjugated
Form Liquid
Purification Method Affinity purification
Storage Buffer Borate buffered saline, pH 8.2.
Storage ConditionsStore at 2-8°C.

Background Information

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.

Protocols

Product Specific Protocols
FC protocol for CD14 antibody 65056-1-IgDownload protocol
Standard Protocols
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