FITC Plus Anti-Human CD14 (UCHM-1)

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• FITC Plus Anti-Human CD14 (UCHM-1) (FITC-65056)

CD14 Monoclonal Antibody for FC

Cat No. FITC-65056 Copied!

Clone No. UCHM-1

Publications (1)

Download datasheet

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Synonyms

UCHM-1, Myeloid cell-specific leucine-rich glycoprotein, Monocyte differentiation antigen CD14, urinary form, Monocyte differentiation antigen CD14, membrane-bound form, CD14 molecule

• 

  FC experiment of human PBMCs using FITC-65056

  1x10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD14 (UCHM-1) (FITC-65056, Clone: UCHM-1) or FITC Plus Mouse IgG2a Isotype Control (C1.18.4) (FITC-65208, Clone: C1.18.4). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of human PBMCs using FITC-65056

  1x10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD14 (UCHM-1) (FITC-65056, Clone: UCHM-1) (red) or FITC Plus Mouse IgG2a Isotype Control (C1.18.4) (FITC-65208, Clone: C1.18.4) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in 100 μl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

FITC-65056 targets CD14 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Thymocytes and peripheral blood lymphocytes from a remission AML patient

• Full Name: CD14 molecule
• Calculated Molecular Weight: 375 aa, 40 kDa
• GenBank Accession Number: BC010507
• Gene Symbol: CD14
• Gene ID (NCBI): 929
• ENSEMBL Gene ID: ENSG00000170458
• RRID: AB_2883746
• Conjugate: FITC Plus Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 495 nm / 524 nm
• Excitation Laser: Blue laser (488 nm)
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: P08571
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.

Publications

What published studies show

FITC-65056 is an Anti-Human CD14 (UCHM-1) monoclonal antibody. It has a total of 1 citation, published in Journal of Bone and Mineral Research (IF 6.5, 2024). The cited study used flow cytometry to investigate monocyte subset imbalances in postmenopausal osteoporosis via single-cell RNA sequencing, placing the research in the fields of bone metabolism, immunology, and aging-related disease.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	FC	J Bone Miner Res; Single-cell RNA sequencing reveals that an imbalance in monocyte subsets rather than changes in gene expression patterns is a feature of postmenopausal osteoporosis; Authors - Lin Tao