FITC Anti-Human CD14 (UCHM-1)

CD14 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

FC

Conjugate

FITC Fluorescent Dye

CloneNo.

UCHM-1

Cat no : FITC-65056

Synonyms

CD14, CD14 molecule



Tested Applications

Positive FC detected inhuman peripheral blood monocytes

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 10 µl per 10^6 cells in a 100 µl suspension or 10 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Product Information

FITC-65056 targets CD14 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Thymocytes and peripheral blood lymphocytes from a remission AML patient
Full Name CD14 molecule
Calculated molecular weight 375 aa, 40 kDa
GenBank accession numberBC010507
Gene symbol CD14
Gene ID (NCBI) 929
Conjugate FITC Fluorescent Dye
Excitation/Emission maxima wavelengths494 nm/520 nm
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.1% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light.

Background Information

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.

Protocols

Product Specific Protocols
FC protocol for FITC CD14 antibody FITC-65056Download protocol
Standard Protocols
Click here to view our Standard Protocols