Tested Applications
| Positive FC detected in | human PBMCs |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 5 ul per 10^6 cells in a 100 µl suspension |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL647-65056 targets CD14 in IF, FC applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Thymocytes and peripheral blood lymphocytes from a remission AML patient Predict reactive species |
| Full Name | CD14 molecule |
| Calculated Molecular Weight | 375 aa, 40 kDa |
| GenBank Accession Number | BC010507 |
| Gene Symbol | CD14 |
| Gene ID (NCBI) | 929 |
| ENSEMBL Gene ID | ENSG00000170458 |
| RRID | AB_2920246 |
| Conjugate | CoraLite® Plus 647 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
| Excitation Laser | Red Laser (633 nm) |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | P08571 |
| Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 647 CD14 antibody CL647-65056 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Anti-Human CD14 (UCHM-1) antibody (Cat# CL647-65056) has 1 citation. It appears in Oncology Letters (2023), where it was used for immunofluorescence (IF) in a study investigating the TLR4/MyD88 signaling pathway in colorectal cancer stem cells following LPS stimulation. The research field encompasses oncology, specifically colorectal cancer biology, and innate immune signaling.
| Species | Application | Title |
|---|---|---|
Oncol Lett Regulation of the migration of colorectal cancer stem cells via the TLR4/MyD88 signaling pathway by the novel surface marker CD14 following LPS stimulation |
Reviews
What customers say
The single review for CL647-65056 is a 3-star rating from a flow cytometry application. The user tested the product on an Attune cytometer with 637 nm excitation, using THP-1 cells (both unstimulated and PMA-stimulated). They noted that the signal was not visible on the RL1 filter (670/14) but only on RL2 (720/30), suggesting the fluorochrome may emit a shifted spectrum. The reviewer indicated the cytometer is calibrated before each use, so the shift likely reflects the dye's emission characteristics rather than instrument error.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Cyril (Verified Customer) (11-28-2024) | We tested the product on the Attune with excitation at 637 nm. I attempted to record the data on the RL1 filter (670/14), but it is only visible on RL2 (720/30). Since the cytometer is checked before each use, it is possible that the fluorochrome emits a shifted spectrum
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