CoraLite®594 Anti-Mouse CD157 Rabbit Recombinant Antibody

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  FC experiment of mouse splenocytes using CL594-98213

  1x10^6 mouse splenocytes were surface stained with 0.25 ug FITC Plus Anti-Mouse CD3 and CoraLite®594 Anti-Mouse CD157 Rabbit RecAb (CL594-98213, Clone: 241822H1) or 0.25 ug CoraLite®594 Rabbit IgG Isotype Control RecAb (CL594-98136, Clone: 240953C9). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using CL594-98213

  1x10^6 mouse splenocytes were surface stained with CoraLite®594 Anti-Mouse CD157 Rabbit RecAb (CL594-98213, Clone: 241822H1) (red) or 0.25 ug CoraLite®594 Rabbit IgG Isotype Control RecAb (CL594-98136, Clone: 240953C9) (blue). Cells were not fixed.

CD157 Uni-rAb^TM Recombinant Antibody for FC

Cat No. CL594-98213

Clone No.241822H1

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

Bp3, Bone marrow stromal antigen 1, Antigen BP3, ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 2, ADP-ribosyl cyclase 2

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-98213 targets CD157 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: bone marrow stromal cell antigen 1
• Calculated Molecular Weight: 35kDa
• Gene Symbol: Bst1
• Gene ID (NCBI): 12182
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q64277
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD157, also known as bone marrow stromal cell antigen 1 (BST-1), or ADP-ribosyl cyclase 2, is a glycosyl phosphotidylinositol-anchored protein that stimulates pre-B-cell growth and has adenosine diphosphate (ADP)-ribosyl cyclase and cyclic ADP-ribose (cADPR) hydrolase activity (PMID: 9473467, 11866528). The two enzymatic activities are responsible for the synthesis and hydrolysis of cADPR, a novel second messenger of calcium release from intracellular calcium stores.