65057-1-Ig
Product Information
65057-1-PBS targets CD16/CD32 in FC applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Host / Isotype | Rat / IgG2a, lambda |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | VH81X Tg B cells and T220 stromal cell line Predict reactive species |
| Full Name | Fc receptor, IgG, low affinity IIb |
| GenBank Accession Number | BC038070 |
| Gene Symbol | CD16/32 |
| Gene ID (NCBI) | 14130 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P08101 |
| Storage Buffer | PBS Only, pH 7.3. |
| Storage Conditions | Store at -80°C. |
Background Information
CD16 (FcγRIII) is a 50-70 kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD32 (FcγRII) is a 40 kD transmembrane glycoprotein that binds to the Fc region of IgG with low affinity. CD32 is present on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Rat anti mouse CD16/32 antibody, clone 93 is specific to the common epitope of CD16 and CD32. It can be used for blocking non-specific binding of immunoglobulins to the Fc receptors by preincubating the cells with the purified antibody at 1 ug per million cells in 100 uL volume for 5-10 minutes prior to immunostaining.
