Anti-Mouse CD16/32 (93)

CD16/32 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2a, lambda

Reactivity

mouse

Applications

IF, FC

Conjugate

Unconjugated

Publications

8

CloneNo.

93

Cat no : 65057-1-Ig

Synonyms

CD32, 93, Fcr 2, FcgRII, Fcgr2b

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Validation Data Gallery

• 

  FC experiment of C57BL/6 mouse splenocytes using 65057-1-Ig

  1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.5 ug Anti-Mouse CD16/32 (65057-1-Ig, Clone: 93) and FITC anti-rat IgG2a Antibody at dilution 1:100, or 0.5 ug Rat IgG2a Isotype Control (2A3) (65209-1-Ig, Clone: 2A3) and FITC anti-rat IgG2a Antibody at dilution 1:100. Cells were co-stained with CoraLite® Plus 647 Anti-Mouse CD3 (17A2) (CL647-65077, Clone: 17A2). Cells were not fixed.

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Tested Applications

• Positive FC detected in: C57BL/6 mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 4 publications below
• FC: See 5 publications below

Product Information

65057-1-Ig targets CD16/32 in IF, FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Cited Reactivity: mouse
• Host / Isotype: Rat / IgG2a, lambda
• Class: Monoclonal
• Type: Antibody
• Immunogen: VH81X Tg B cells and T220 stromal cell line
• Full Name: Fc receptor, IgG, low affinity IIb
• GenBank Accession Number: BC038070
• Gene Symbol: CD16/32
• Gene ID (NCBI): 14130
• RRID: AB_2918365
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD16 (FcγRIII) is a 50-70 kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD32 (FcγRII) is a 40 kD transmembrane glycoprotein that binds to the Fc region of IgG with low affinity. CD32 is present on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Rat anti mouse CD16/32 antibody, clone 93 is specific to the common epitope of CD16 and CD32. It can be used for blocking non-specific binding of immunoglobulins to the Fc receptors by preincubating the cells with the purified antibody at 1 ug per million cells in 100 uL volume for 5-10 minutes prior to immunostaining.

Publications

Species	Application	Title
	FC	Adv Mater; Ultrasound Imaging of Tumor Vascular CD93 with MMRN2 Modified Microbubbles for Immune Microenvironment Prediction; Authors - Dingyi Wang
mouse	FC	Front Microbiol; Response to HIV-1 gp160-carrying recombinant virus HSV-1 and HIV-1 VLP combined vaccine in BALB/c mice; Authors - Beibei Zhang
mouse	IF	Cell Signal; OTUB1's role in promoting OSCC development by stabilizing RACK1 involves cell proliferation, migration, invasion, and tumor-associated macrophage M1 polarization; Authors - Yunyun Li
mouse	IF	Exp Brain Res; Silencing CXCL16 alleviate neuroinflammation and M1 microglial polarization in mouse brain hemorrhage model and BV2 cell model through PI3K/AKT pathway; Authors - Lv Dingyi
mouse	FC	Bioact Mater; Reprogramming exosomes for immunity-remodeled photodynamic therapy against non-small cell lung cancer; Authors - Jiao Guo
mouse	IF,FC	J Nanobiotechnology; Bioengineered stem cell membrane functionalized nanoparticles combine anti-inflammatory and antimicrobial properties for sepsis treatment; Authors - Lu Lu