Tested Applications
| Positive FC detected in | C57BL/6 mouse splenocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.5 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
65057-1-Ig targets CD16/CD32 in IF, FC, Blocking assay applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rat / IgG2a, lambda |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
VH81X Tg B cells and T220 stromal cell line Predict reactive species |
| Full Name | Fc receptor, IgG, low affinity IIb |
| GenBank Accession Number | BC038070 |
| Gene Symbol | CD16/32 |
| Gene ID (NCBI) | 14130 |
| RRID | AB_2918365 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P08101 |
| Storage Buffer | PBS with 0.09% sodium azide, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Stable for one year after shipment. |
Background Information
CD16 (FcγRIII) is a 50-70 kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD32 (FcγRII) is a 40 kD transmembrane glycoprotein that binds to the Fc region of IgG with low affinity. CD32 is present on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Rat anti mouse CD16/32 antibody, clone 93 is specific to the common epitope of CD16 and CD32. It can be used for blocking non-specific binding of immunoglobulins to the Fc receptors by preincubating the cells with the purified antibody at 1 ug per million cells in 100 uL volume for 5-10 minutes prior to immunostaining.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CD16/CD32 antibody 65057-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-CD93 antibody (Cat# 65057-1-Ig) has 24 citations published in journals including Nature Communications, Advanced Materials, Advanced Science, EMBO Journal, Brain, Behavior, and Immunity, and Cell Death & Disease. Research fields span tumor immunology, macrophage/microglia polarization, neuroinflammation, sepsis, and multiple cancer types (lung, colorectal, pancreatic, ovarian). Primary applications are flow cytometry, immunofluorescence, and blocking assays, predominantly in mouse models.
| Species | Application | Title |
|---|---|---|
Adv Mater Ultrasound Imaging of Tumor Vascular CD93 with MMRN2 Modified Microbubbles for Immune Microenvironment Prediction | ||
Bioact Mater Reprogramming exosomes for immunity-remodeled photodynamic therapy against non-small cell lung cancer | ||
Nat Commun Cu/Zn/histidine supramolecular assemblies with optimized Cu catalytic sites as an alternative of superoxide dismutase. | ||
J Nanobiotechnology Bioengineered stem cell membrane functionalized nanoparticles combine anti-inflammatory and antimicrobial properties for sepsis treatment | ||
Genes Dis Identification of PLXNC1 as a novel biomarker for consensus molecular subtype 4 in colorectal cancer. | ||
Adv Sci (Weinh) Enhancing Chemotherapy-Related Immune Responses via Bioorthogonal Metabolic Engineering-Driven Tumor Exosomes Elimination. |
