Anti-Mouse CD16/32 (93)

CD16/32 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2a, lambda

Reactivity

Mouse and More (1)

Applications

IF, FC

Conjugate

Unconjugated

CloneNo.

93

Cat no : 65057-1-Ig

Synonyms

AI528646, CD16/32, CD32, F630109E10Rik, Fc[g]RII, Fcgr2, Fcgr2a, Fcgr2b, FcgRII, Fcr 2, Fcr 3, Ly 17, Ly m20, LyM 1



Tested Applications

Positive FC detected inC57BL/6 mouse splenocytes

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65057-1-Ig targets CD16/32 in IF, FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Cited Reactivitymouse
Host / Isotype Rat / IgG2a, lambda
Class Monoclonal
Type Antibody
Immunogen VH81X Tg B cells and T220 stromal cell line
Full Name Fc receptor, IgG, low affinity IIb
GenBank Accession NumberBC038070
Gene Symbol CD16/32
Gene ID (NCBI) 14130
RRIDAB_2918365
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

CD16 (FcγRIII) is a 50-70 kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD32 (FcγRII) is a 40 kD transmembrane glycoprotein that binds to the Fc region of IgG with low affinity. CD32 is present on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Rat anti mouse CD16/32 antibody, clone 93 is specific to the common epitope of CD16 and CD32. It can be used for blocking non-specific binding of immunoglobulins to the Fc receptors.

Protocols

Product Specific Protocols
FC protocol for CD16/32 antibody 65057-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
FC

Adv Mater

Ultrasound Imaging of Tumor Vascular CD93 with MMRN2 Modified Microbubbles for Immune Microenvironment Prediction

Authors - Dingyi Wang
mouseFC

Front Microbiol

Response to HIV-1 gp160-carrying recombinant virus HSV-1 and HIV-1 VLP combined vaccine in BALB/c mice

Authors - Beibei Zhang
mouseIF

Cell Signal

OTUB1's role in promoting OSCC development by stabilizing RACK1 involves cell proliferation, migration, invasion, and tumor-associated macrophage M1 polarization

Authors - Yunyun Li
mouseIF

Exp Brain Res

Silencing CXCL16 alleviate neuroinflammation and M1 microglial polarization in mouse brain hemorrhage model and BV2 cell model through PI3K/AKT pathway

Authors - Lv Dingyi
mouseIF,FC

J Nanobiotechnology

Bioengineered stem cell membrane functionalized nanoparticles combine anti-inflammatory and antimicrobial properties for sepsis treatment

Authors - Lu Lu
mouseFC

Stem Cells Dev

Deer antler reserve mesenchyme cell-conditioned medium reduces the destruction of periodontitis in mice

Authors - Hongbing Lin