Anti-Mouse CD16/32 (93)

CD16/32 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2a

Reactivity

Mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

93

Cat no : 65057-1-Ig

Synonyms

AI528646, CD16/32, CD32, F630109E10Rik, Fc[g]RII, Fcgr2, Fcgr2a, Fcgr2b, FcgRII, Fcr 2, Fcr 3, Ly 17, Ly m20, LyM 1



Tested Applications

Positive FC detected inmouse splenocytes
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®647 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

65057-1-Ig targets CD16/32 in FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Rat / IgG2a
Class Monoclonal
Type Antibody
Immunogen VH81X Tg B cells and T220 stromal cell line
Full Name Fc receptor, IgG, low affinity IIb
GenBank accession numberBC038070
Gene symbol Fcgr2b
Gene ID (NCBI) 14130
Conjugate Unconjugated
Form Liquid
Purification Method Affinity purification
Storage Buffer Borate buffered saline, pH 8.2.
Storage ConditionsStore at 2-8°C.

Background Information

CD16 (FcγRIII) is a 50-70 kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD32 (FcγRII) is a 40 kD transmembrane glycoprotein that binds to the Fc region of IgG with low affinity. CD32 is present on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes.

Protocols

Product Specific Protocols
FC protocol for CD16/32 antibody 65057-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols