FITC Plus Anti-Human CD160 Rabbit Recombinant Antibody

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  FC experiment of human peripheral blood leukocytes using FITC-98111

  1x10^6 human peripheral blood leukocytes were surface stained with 5 ul FITC Plus Anti-Human CD160 Rabbit RecAb (FITC-98111, Clone: 241415F2), or FITC Plus Rabbit IgG Isotype Control RecAb (FITC-98136, Clone: 240953C9). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human peripheral blood leukocytes using FITC-98111

  1x10^6 human peripheral blood leukocytes were surface stained with 5 ul FITC Plus Anti-Human CD160 Rabbit RecAb (FITC-98111, Clone: 241415F2)(red) or FITC Plus Rabbit IgG Isotype Control RecAb (FITC-98136, Clone: 240953C9) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

CD160 Uni-rAb^TM Recombinant Antibody for FC

Cat No. FITC-98111

Clone No.241415F2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

241415F2, BY55, CD160 antigen, CD160 antigen, soluble form, CD160 molecule

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Tested Applications

• Positive FC detected in: human peripheral blood leukocytes

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

FITC-98111 targets CD160 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: CD160 molecule
• Calculated Molecular Weight: 20 kDa
• GenBank Accession Number: BC014465
• Gene Symbol: CD160
• Gene ID (NCBI): 11126
• Conjugate: FITC Plus Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 495 nm / 524 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O95971
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD160 is a glycosylphosphatidylinositol (GPI)-anchored protein that is expressed on almost all intestinal intraepithelial lymphocytes (IELs), gamma delta T cells, CD56 (dim) CD16+ cytotoxic NK cells, and a minor subset of CD4+ and CD8+ T cells (PMID: 31332204; 25179432). The binding of CD160 to both classical and non-classical MHC I enhances NK and CD8+ cytotoxic T lymphocyte functions (PMID: 25179432). CD160 is a ligand for HVEM (herpes virus entry mediator) and the binding of CD160 to HVEM inhibits T cell activity (PMID: 19426226).