CD22 Monoclonal antibody

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  WB analysis of Ramos using 66103-1-Ig

  Ramos, Daudi, and Raji cells were subjected to SDS PAGE followed by western blot with 66103-1-Ig (CD22 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 66103-1-Ig

  Untreated and PNGase F-treated lysates of Raji cells were subjected to SDS PAGE followed by western blot with 66103-1-Ig (CD22 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).

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  IHC staining of human tonsillitis using 66103-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66103-1-Ig (CD22 antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 66103-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66103-1-Ig (CD22 antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 66103-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66103-1-Ig (CD22 antibody) at dilution of 1:20000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendicitis using 66103-1-Ig

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66103-1-Ig (CD22 antibody) at dilution of 1:6400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendicitis using 66103-1-Ig

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66103-1-Ig (CD22 antibody) at dilution of 1:6400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human tonsillitis using 66103-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD22 antibody (66103-1-Ig, Clone: 7A2F1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of human tonsillitis using 66103-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD22 antibody (66103-1-Ig, Clone: 7A2F1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of Raji using 66103-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD22 antibody (66103-1-Ig, Clone: 7A2F1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

CD22 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Cat No. 66103-1-Ig

Clone No.7A2F1

Publications(1)

Reviews (1)

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Synonyms

7A2F1, B cell receptor CD22, B-cell receptor CD22, BL-CAM, B-lymphocyte cell adhesion molecule

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Tested Applications

• Positive WB detected in: Ramos cells, Raji cells, Daudi cells
• Positive IHC detected in: human tonsillitis tissue, human appendicitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human tonsillitis tissue, Raji cells
• Positive IF/ICC detected in: Raji cells

Recommended dilution

• Western Blot (WB): WB : 1:2500-1:10000
• Immunohistochemistry (IHC): IHC : 1:10000-1:40000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

66103-1-Ig targets CD22 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD22 fusion protein Ag17986
• Full Name: CD22 molecule
• Calculated Molecular Weight: 847 aa, 95 kDa
• Observed Molecular Weight: 135 kDa
• GenBank Accession Number: BC109306
• Gene Symbol: CD22
• Gene ID (NCBI): 933
• RRID: AB_2881502
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P20273
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD22, also known as Siglec-2 (sialic acid binding Ig-like lectin 2) or BL-CAM (B-lymphocyte cell adhesion molecule), is a 130-140 kDa, B-cell restricted, type I transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. The expression of CD22 is developmentally regulated. It is expressed at low levels in the cytoplasm of pro-B and pre-B cells and present on the cell surface only at mature stages of B-cell differentiation. Cell surface expression is lost during terminal differentiation into plasma cell and after B-cell activation. CD22 is an inhibitory receptor for B-cell receptor (BCR) signalling, preferentially binds to alpha-2,6-linked sialic acid and mediates B-cell B-cell interactions. It plays a crucial role in activation and differentiation of the B-cell.

Publications

Species	Application	Title
human	IF	J Biomed Res; Causal genetic regulation of DNA replication on immune microenvironment in colorectal tumorigenesis: evidenced by an integrated approach of trans-omics and GWAS; Authors - Sumeng Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Emma (Verified Customer) (11-29-2021)

Works well by IF on FFPE tissue. Antigen retrieval Tris-EDTA pH 9.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Prostate

CD22 Antibody Immunofluorescence validation (1:100 dilution) in Prostate (Cat no:66103-1-Ig)