CD22 Monoclonal antibody
CD22 Monoclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2a
WB, IHC, IF, ELISA
Cat no : 66103-1-Ig
Validation Data Gallery
|Positive WB detected in||Ramos cells, Raji cells, Daudi cells|
|Positive IHC detected in||human tonsillitis tissue, human appendicitis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||human tonsillitis tissue, Raji cells|
|Western Blot (WB)||WB : 1:2500-1:10000|
|Immunohistochemistry (IHC)||IHC : 1:10000-1:40000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 66103-1-Ig is CD22 Fusion Protein expressed in E. coli.
|Host / Isotype||Mouse / IgG2a|
|Immunogen||CD22 fusion protein Ag17986|
|Full Name||CD22 molecule|
|Calculated molecular weight||847 aa, 95 kDa|
|Observed molecular weight||135 kDa|
|GenBank accession number||BC109306|
|Gene ID (NCBI)||933|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CD22, also known as Siglec-2 (sialic acid binding Ig-like lectin 2) or BL-CAM (B-lymphocyte cell adhesion molecule), is a 130-140 kDa, B-cell restricted, type I transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. The expression of CD22 is developmentally regulated. It is expressed at low levels in the cytoplasm of pro-B and pre-B cells and present on the cell surface only at mature stages of B-cell differentiation. Cell surface expression is lost during terminal differentiation into plasma cell and after B-cell activation. CD22 is an inhibitory receptor for B-cell receptor (BCR) signalling, preferentially binds to alpha-2,6-linked sialic acid and mediates B-cell B-cell interactions. It plays a crucial role in activation and differentiation of the B-cell.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Emma (Verified Customer) (11-29-2021)
Works well by IF on FFPE tissue. Antigen retrieval Tris-EDTA pH 9.