CoraLite®488-conjugated CD22 Monoclonal antibody
CD22 Monoclonal Antibody for IF
Host / Isotype
Mouse / IgG2a
CoraLite®488 Fluorescent Dye
Cat no : CL488-66103
|Positive IF detected in
|human tonsillitis tissue
|IF : 1:50-1:500
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
The immunogen of CL488-66103 is CD22 Fusion Protein expressed in E. coli.
|Host / Isotype
|Mouse / IgG2a
|CD22 fusion protein Ag17986
|Calculated molecular weight
|847 aa, 95 kDa
|GenBank accession number
|Gene ID (NCBI)
|CoraLite®488 Fluorescent Dye
|Excitation/Emission maxima wavelengths
|491 nm / 516 nm
|Protein A purification
|PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
|Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
CD22, also known as Siglec-2 (sialic acid binding Ig-like lectin 2) or BL-CAM (B-lymphocyte cell adhesion molecule), is a 130-140 kDa, B-cell restricted, type I transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. The expression of CD22 is developmentally regulated. It is expressed at low levels in the cytoplasm of pro-B and pre-B cells and present on the cell surface only at mature stages of B-cell differentiation. Cell surface expression is lost during terminal differentiation into plasma cell and after B-cell activation. CD22 is an inhibitory receptor for B-cell receptor (BCR) signalling, preferentially binds to alpha-2,6-linked sialic acid and mediates B-cell B-cell interactions. It plays a crucial role in activation and differentiation of the B-cell. And this antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.