CD23/Fc epsilon RII Recombinant antibody

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  WB analysis of mouse spleen using 84212-3-RR

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 84212-3-RR (CD23/Fc epsilon RII antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse spleen using 84212-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84212-3-RR (CD23/Fc epsilon RII antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 84212-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84212-3-RR (CD23/Fc epsilon RII antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 84212-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84212-3-RR against Mouse CD23/Fc epsilon RII were performed. The affinity constant is 0.168 nM.

CD23/Fc epsilon RII Uni-rAb^TM Recombinant Antibody for WB, IHC, ELISA

Cat No. 84212-3-RR

Clone No.241385C9

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, ELISA

Synonyms

241385C9, CD23, Fc-epsilon-RII, Fcer2, Fcer2a

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Tested Applications

• Positive WB detected in: mouse spleen tissue
• Positive IHC detected in: mouse spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84212-3-RR targets CD23/Fc epsilon RII in WB, IHC, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CD23/Fc epsilon RII fusion protein Eg1295
• Full Name: Fc receptor, IgE, low affinity II, alpha polypeptide
• Calculated Molecular Weight: 38kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: EDL21948.1
• Gene Symbol: Fcer2a
• Gene ID (NCBI): 14128
• RRID: AB_3671769
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P20693-1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD23/Fc ε RII) is a low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21, involved in B cell differentiation and IgE regulation. Two forms of Fc ε RII (Fc ε RIIa and Fc ε RIIb) were found to exist, which differ in structure only in the N-terminal cytoplasmic region, and Fc ε RIIa and Fc ε RIIb play roles in effector cells of B-cell and IgE-mediated immunity, respectively. On B cells, initiates IgE-dependent antigen uptake and presentation to T cells. On macrophages, after IgE binding and antigen cross-linking, intracellular killing of parasites is induced by activation of the L-arginine-nitric oxide pathway.