Anti-Human CD244 (2B4.69)

CD244 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

2B4.69

Cat no : 65270-1-Ig

Synonyms

2B4, CD244, h2B4, NAIL, NKR2B4, SLAMF4

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Validation Data Gallery

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  FC experiment of human PBMCs using 65270-1-Ig

  1X10^6 human PBMCs were surface co-stained with CL750 Anti-Human CD56 and 0.2 ug Anti-Human CD244 (65270-1-Ig, Clone:2B4.69) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000, or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65270-1-Ig

  1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD244 (65270-1-Ig, Clone:2B4.69) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000, or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65270-1-Ig targets CD244 in FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: N/A
• Full Name: CD244 molecule, natural killer cell receptor 2B4
• GenBank Accession Number: BC028073
• Gene Symbol: CD244
• Gene ID (NCBI): 51744
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: 2-8°C.

Background Information

CD244, also known as SLAMF4 or 2B4, is a type I transmembrane glycoprotein belonging to the signaling lymphocyte activation molecule (SLAM) family. It consists of an extracellular segment with two immunoglobulin (Ig)-like domains, a transmembrane region, and a cytoplasmic domain containing tyrosine-based motifs (PMID: 9841922; 30546369). CD244 is present on natural killer (NK) cells, γδ T cells, a subset of CD8+ T cells, monocytes, basophils, dendritic cells, and myeloid-derived suppressor cells (MDSCs) (PMID: 30546369). It binds to CD48 with high affinity and transmits stimulatory or inhibitory signals that regulate immune function (PMID: 9841922; 18523281).