CoraLite® Plus 555 Anti-Human CD244 (2B4.69)

• 

  FC experiment of human PBMCs using CL555-65270

  1X10^6 human PBMCs were surface co-stained with CL750 Anti-Human CD56 and 5 ul CoraLite® Plus 555 Anti-Human CD244 (CL555-65270, Clone:2B4.69), or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using CL555-65270

  1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 555 Anti-Human CD244 (CL555-65270, Clone:2B4.69), or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

CD244 Monoclonal Antibody for FC

Cat No. CL555-65270

Clone No.2B4.69

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC

Synonyms

2B4, CD244, h2B4, NAIL, NKR2B4, SLAMF4

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in 100 μl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

CL555-65270 targets CD244 in FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  N/A

• Full Name: CD244 molecule, natural killer cell receptor 2B4
• GenBank Accession Number: BC028073
• Gene Symbol: CD244
• Gene ID (NCBI): 51744
• RRID: AB_3084619
• Conjugate: CoraLite® Plus 555 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 554 nm / 570 nm
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: Q9BZW8
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD244, also known as SLAMF4 or 2B4, is a type I transmembrane glycoprotein belonging to the signaling lymphocyte activation molecule (SLAM) family. It consists of an extracellular segment with two immunoglobulin (Ig)-like domains, a transmembrane region, and a cytoplasmic domain containing tyrosine-based motifs (PMID: 9841922; 30546369). CD244 is present on natural killer (NK) cells, γδ T cells, a subset of CD8+ T cells, monocytes, basophils, dendritic cells, and myeloid-derived suppressor cells (MDSCs) (PMID: 30546369). It binds to CD48 with high affinity and transmits stimulatory or inhibitory signals that regulate immune function (PMID: 9841922; 18523281).