CoraLite® Plus 405 Anti-Mouse B7-H3/CD276 (MJ18)

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  FC experiment of BALB/c mouse peritoneal macrophages using CL405-65221

  1X10^6 BALB/c mouse peritoneal macrophages were surface stained with 0.5 ug CoraLite® Plus 405 Anti-Mouse CD276 (CL405-65221, Clone: MJ18) or unstained. Cells were not fixed.

B7-H3/CD276 Monoclonal Antibody for FC

Cat No. CL405-65221

Clone No.MJ18

Host / Isotype

Rat / IgG1, kappa

Reactivity

mouse

Applications

FC

Synonyms

CD276, CD276 antigen, B7RP 2, B7-H3, B7h3

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Tested Applications

• Positive FC detected in: BALB/c mouse peritoneal macrophages

Recommended dilution

• Flow Cytometry (FC): FC : 0.5 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL405-65221 targets B7-H3/CD276 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rat / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Mouse IgG2a Fc-CD276 (aa 1-242) fusion protein

• Full Name: CD276 antigen
• GenBank Accession Number: BC019436
• Gene Symbol: Cd276
• Gene ID (NCBI): 102657
• RRID: AB_3672392
• Conjugate: CoraLite® Plus 405 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 399 nm / 422 nm
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: Q8VE98
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD276 (also known as B7-H3) is a type I transmembrane protein expressed on many tissues and cell types. CD276 is a 100-kDa glycoprotein that belongs to the B7 immunoregulatory family and participates in the regulation of T-cell-mediated immune response probably by functioning as both a T cell costimulator and coinhibitor (PMID: 25567370; 20696859). Overexpressed on a wide range of human solid cancers, CD276 has been implicated in cancer progression and metastasis and becomes an attractive target for cancer immunotherapy (PMID: 27208063).