CoraLite® Plus 488 Anti-Mouse CD276 (MJ18)

CD276 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG1, kappa

Reactivity

Mouse

Applications

FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

MJ18

Cat no : CL488-65221

Synonyms

6030411F23Rik, AU016588, B7h3, B7RP 2, Cd276, CD276 antigen

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Validation Data Gallery

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  FC experiment of BALB/c mouse peritoneal macrophages using CL488-65221

  1X10^6 mouse peritoneal macrophages were surface stained with 0.5 ug CoraLite® Plus 488 Anti-Mouse CD276 (CL488-65221, Clone: MJ18) or unstained. Cells were not fixed.

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Tested Applications

• Positive FC detected in: mouse peritoneal macrophages

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-65221 targets CD276 in FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Rat / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Mouse IgG2a Fc-CD276 (aa 1-242) fusion protein
• Full Name: CD276 antigen
• GenBank Accession Number: BC019436
• Gene Symbol: Cd276
• Gene ID (NCBI): 102657
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD276 (also known as B7-H3) is a type I transmembrane protein expressed on many tissues and cell types. CD276 is a 100-kDa glycoprotein that belongs to the B7 immunoregulatory family and participates in the regulation of T-cell-mediated immune response probably by functioning as both a T cell costimulator and coinhibitor (PMID: 25567370; 20696859). Overexpressed on a wide range of human solid cancers, CD276 has been implicated in cancer progression and metastasis and becomes an attractive target for cancer immunotherapy (PMID: 27208063).