FITC Plus Anti-Human CD3 (OKT3)

CD3 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2a, kappa

Reactivity

human and More (1)

Applications

IF, FC

Conjugate

FITC Plus Fluorescent Dye

Publications

6

CloneNo.

OKT3

Cat no : FITC-65133

Synonyms

CD3E, CD3 epsilon, T-cell surface antigen T3/Leu-4 epsilon chain, T3E, OKT3

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Validation Data Gallery

• 

  FC experiment of human PBMCs using FITC-65133

  1X10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD3 (FITC-65133, Clone:OKT3), or Mouse IgG2a Isotype Control. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using FITC-65133

  1X10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD3 (FITC-65133, Clone:OKT3), or Mouse IgG2a Isotype Control. Cells were not fixed. Lymphocytes were gated.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below
• FC: See 4 publications below

Product Information

FITC-65133 targets CD3 in IF, FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Human T cells
• Full Name: CD3e molecule, epsilon (CD3-TCR complex)
• Calculated Molecular Weight: 207 aa, 23 kDa
• GenBank Accession Number: BC049847
• Gene Symbol: CD3
• Gene ID (NCBI): 916
• RRID: AB_2883794
• Conjugate: FITC Plus Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 495 nm / 524 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).

Publications

Species	Application	Title
		Int J Pharm; A self-assembly active nanomodulator based on berberine for photothermal immunotherapy of breast cancer via dual regulation of immune suppression; Authors - Jiang Sun
mouse	FC	ACS Biomater Sci Eng; Fe3O4@R837 Nanoplatform Enhances Chemical Dynamic Therapy and Immunotherapy: Integrated Transcriptomic Analysis Reveals Key Genes in Breast Cancer Prognosis; Authors - Shichao Zhang
mouse	FC	Adv Healthc Mater; Harnessing Dual Phototherapy and Immune Activation for Cancer Treatment: The Development and Application of BODIPY@F127 Nanoparticles; Authors - Yang Peng
human	FC	J Biol Eng; Construction of bispecific antibodies by specific pairing between the heavy chain and the light chain using removable SpyCatcher/SnoopCatcher units; Authors - Jyunna Yoshida
	IF	J Med Chem; Platinum(IV) Complexes as Inhibitors of STAT3 and Regulators of the Tumor Microenvironment To Control Breast Cancer; Authors - Linxiang Cai
human	FC	Sci Rep; Construction of IgG-Fab2 bispecific antibody via intein-mediated protein trans-splicing reaction; Authors - Risa Yamada