|Positive FC detected in||human PBMCs|
|This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.|
|Sample-dependent, check data in validation data gallery|
PE-65133 targets CD3 in FC applications and shows reactivity with Human samples.
|Cited Reactivity||human, mouse|
|Host / Isotype||Mouse / IgG2a, kappa|
|Full Name||CD3e molecule, epsilon (CD3-TCR complex)|
|Calculated molecular weight||207 aa, 23 kDa|
|GenBank accession number||BC049847|
|Gene ID (NCBI)||916|
|Conjugate||PE Fluorescent Dye|
|Excitation/Emission maxima wavelengths||496 nm, 565 nm / 578 nm|
|Purification Method||Affinity purification|
|Storage Buffer||PBS with 0.09% sodium azide and 0.5% BSA.|
|Storage Conditions||Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.|
CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Amelia (Verified Customer) (12-19-2022)
CD3 antibody worked as it should, good separation of positive/negative population.