Tested Applications
| Positive FC detected in | human PBMCs |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 5 ul per 10^6 cells in 100 μl suspension |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
PE-65133 targets CD3 in FC applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Human T cells Predict reactive species |
| Full Name | CD3e molecule, epsilon (CD3-TCR complex) |
| Calculated Molecular Weight | 207 aa, 23 kDa |
| GenBank Accession Number | BC049847 |
| Gene Symbol | CD3 |
| Gene ID (NCBI) | 916 |
| ENSEMBL Gene ID | ENSG00000198851 |
| RRID | AB_2883916 |
| Conjugate | PE Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 496 nm, 565 nm / 578 nm |
| Excitation Laser | Blue Laser (488 nm), Green Laser (532 nm), Yellow-Green Laser (561 nm) |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | P07766 |
| Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for PE CD3 antibody PE-65133 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
PE-65133 is an Anti-Human CD3 (OKT3) monoclonal antibody with 3 citations. The cited journals are Advanced Science, Pharmacological Research, and DNA and Cell Biology. Research fields include tumor immunotherapy (mRNA-encoded bispecific antibody delivery), T cell-mediated alloimmunity and immunosuppression, and rheumatoid arthritis involving Th17/Treg cell imbalance. All three studies applied flow cytometry (FC) as the tested application.
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Lipid Nanoparticle Delivery System for mRNA Encoding B7H3-redirected Bispecific Antibody Displays Potent Antitumor Effects on Malignant Tumors | ||
Pharmacol Res CX-5461 is a potent immunosuppressant which inhibits T cell-mediated alloimmunity via p53-DUSP5. | ||
DNA Cell Biol Upregulated Expression of microRNA-16 Correlates with Th17/Treg Cell Imbalance in Patients with Rheumatoid Arthritis. |
Reviews
What customers say
One reviewer rated PE-65133 four out of five stars, noting that the CD3 antibody performed as expected with good separation of positive and negative populations in flow cytometry. Overall, the feedback is positive regarding the antibody's functionality and staining quality.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Amelia (Verified Customer) (12-19-2022) | CD3 antibody worked as it should, good separation of positive/negative population.
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