PE Anti-Human CD3 (OKT3)

CD3 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

Human and More (1)

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

OKT3

Cat no : PE-65133

Synonyms

CD3, CD3E, T3E, TCRE



Tested Applications

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD3 Ready-To-Use IHC Kit. CD3 primary antibody included.

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Published Applications

FCSee 1 publications below

Product Information

PE-65133 targets CD3 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivitymouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen N/A
Full Name CD3e molecule, epsilon (CD3-TCR complex)
Calculated molecular weight 207 aa, 23 kDa
GenBank accession numberBC049847
Gene symbol CD3E
Gene ID (NCBI) 916
Conjugate PE Fluorescent Dye
Excitation/Emission maxima wavelengths496 nm, 565 nm / 578 nm
Form Liquid
Purification Method Affinity purification
Storage Buffer Phosphate based buffer with 0.09% sodium azide and 0.1% gelatin, pH 7.2.
Storage ConditionsStore at 2-8°C. Avoid exposure to light.

Background Information

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).

Protocols

Product Specific Protocols
FC protocol for PE CD3 antibody PE-65133Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseFC

Pharmacol Res

CX-5461 is a potent immunosuppressant which inhibits T cell-mediated alloimmunity via p53-DUSP5.

Authors - Guopin Pan