Rat CD31/PECAM-1 Recombinant antibody, PBS Only

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  WB analysis using 85898-4-RR (same clone as 85898-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85898-4-RR (CD31/PECAM-1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85898-4-PBS in a different storage buffer formulation.

• 

  WB analysis of rat spleen using 85898-4-RR (same clone as 85898-4-PBS)

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 85898-4-RR (CD31/PECAM-1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85898-4-PBS in a different storage buffer formulation.

• 

  IHC staining of rat liver using 85898-4-RR (same clone as 85898-4-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 85898-4-RR (CD31/PECAM-1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85898-4-PBS in a different storage buffer formulation.

• 

  IHC staining of rat liver using 85898-4-RR (same clone as 85898-4-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 85898-4-RR (CD31/PECAM-1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85898-4-PBS in a different storage buffer formulation.

CD31/PECAM-1 Uni-rAb^TM Recombinant Antibody for WB, IHC, Indirect ELISA

Cat No. 85898-4-PBS

Clone No.250372F8

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

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Product Information

85898-4-PBS targets CD31/PECAM-1 in WB, IHC, Indirect ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CD31/PECAM-1 fusion protein Eg1456
• Full Name: platelet/endothelial cell adhesion molecule 1
• Calculated Molecular Weight: 76kDa
• Observed Molecular Weight: 100-130 kDa
• GenBank Accession Number: NP_113779.1
• Gene Symbol: Pecam1
• Gene ID (NCBI): 29583
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q3SWT0
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).