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Tested Applications
| Positive FC detected in | human PBMCs |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 5 ul per 10^6 cells in 100 μl suspension |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CR-65272 targets CD33 in FC applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
fusion protein Predict reactive species |
| Full Name | CD33 molecule |
| Calculated Molecular Weight | 364 aa, 40 kDa |
| GenBank Accession Number | BC028152 |
| Gene Symbol | CD33 |
| Gene ID (NCBI) | 945 |
| RRID | AB_3084992 |
| Conjugate | Cardinal Red™ Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 592 nm / 611 nm |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | P20138 |
| Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD33, also referred to as Siglec-3, is a 67-kDa glycosylated transmembrane protein of sialic acid-binding immunoglobulin-like lactic (siglec) family. CD33 is expressed on monocytes, myeloid progenitors, granulocytes, mast cells, some T cells. It may mediate cell-to-cell adhesion and act as a receptor that inhibits the proliferation of normal and leukemic myeloid cells.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for Cardinal Red™ CD33 antibody CR-65272 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
