Tested Applications
| Positive FC detected in | human PBMCs |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 5 ul per 10^6 cells in 100 μl suspension |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-65272 targets CD33 in FC applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
N/A Predict reactive species |
| Full Name | CD33 molecule |
| Calculated Molecular Weight | 364 aa, 40 kDa |
| GenBank Accession Number | BC028152 |
| Gene Symbol | CD33 |
| Gene ID (NCBI) | 945 |
| RRID | AB_3084190 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Excitation Laser | Blue laser (488 nm) |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | P20138 |
| Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD33, also referred to as Siglec-3, is a 67-kDa glycosylated transmembrane protein of sialic acid-binding immunoglobulin-like lactic (siglec) family. CD33 is expressed on monocytes, myeloid progenitors, granulocytes, mast cells, some T cells. It may mediate cell-to-cell adhesion and act as a receptor that inhibits the proliferation of normal and leukemic myeloid cells.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 CD33 antibody CL488-65272 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
CL488-65272 is an Anti-Human CD33 (WM53) monoclonal antibody conjugated with CoraLite® Plus 488. It has 1 total citation, published in Cell Death & Disease (IF: 12.2). The cited study investigated granulocyte differentiation arrest in HAX1-deficient cells as a model of Kostmann disease, linking it to defective lipid droplet autophagy and impaired fatty acid uptake. The research field encompasses cell death, granulopoiesis, inherited bone marrow failure syndromes, and lipid metabolism.
| Species | Application | Title |
|---|---|---|
Cell Death Dis Granulocyte differentiation arrest in HAX1-deficient cells, demonstrated in a new in vitro model of a certain phenotypic aspects of Kostmann disease, is caused by ineffective lipid droplet autophagy and fatty acids uptake. |
