Anti-Human CD4 (OKT4) Rabbit IgG Recombinant Antibody, PBS Only

• 

  FC experiment of human PBMCs using 65505-1-PBS

  1X10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD4 Rabbit Recombinant Antibody (65505-1-PBS, Clone:OKT4) or Rabbit IgG control PolyAb, and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were then stained with CL488 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65505-1-RR (same clone as 65505-1-PBS)

  1X10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD4 Rabbit Recombinant Antibody (65505-1-RR, Clone:OKT4) or Rabbit IgG control PolyAb, and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were then stained with CL488 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65505-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65505-1-RR (same clone as 65505-1-PBS)

  1X10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD4 Rabbit Recombinant Antibody (65505-1-RR, Clone:OKT4) or Rabbit IgG control PolyAb, and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65505-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65505-1-PBS

  1X10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD4 Rabbit Recombinant Antibody (65505-1-PBS, Clone:OKT4) or Rabbit IgG control PolyAb, and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65505-1-PBS

  1X10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD4 Rabbit Recombinant Antibody (65505-1-PBS, Clone:OKT4) and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (left) or 0.1 ug Anti-Human CD4 Mouse Monoclonal Antibody (Clone:OKT4) and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000 (center and right). Cells were then stained with CL488 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65505-1-RR (same clone as 65505-1-PBS)

  1X10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD4 Rabbit Recombinant Antibody (65505-1-RR, Clone:OKT4) and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (left) or 0.1 ug Anti-Human CD4 Mouse Monoclonal Antibody (Clone:OKT4) and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000 (center and right). Cells were then stained with CL488 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65505-1-PBS in a different storage buffer formulation.

CD4 Uni-rAb^® Recombinant Antibody for FC

Cat No. 65505-1-PBS

Clone No.OKT4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

CD4 molecule, CD4mut, OKT4, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 0.1 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

65505-1-PBS targets CD4 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Human peripheral T cells

• Full Name: CD4 molecule
• Calculated Molecular Weight: 55 kDa
• GenBank Accession Number: BC025782
• Gene Symbol: CD4
• Gene ID (NCBI): 920
• ENSEMBL Gene ID: ENSG00000010610
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: P01730
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C. Stable for one year after shipment.

Background Information

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).