Anti-Human CD4 Rabbit scFv Antibody

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• Anti-Human CD4 Rabbit scFv Antibody (SF98042-1-RR)

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  SDS-PAGE of SF98042-1-RR

  Purity of Recombinant ScFv antibody(Cat No.:SF98042-1-RR) was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and condition and stained using Coomassie blue.

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  FC experiment of human PBMCs using SF98042-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug CD4 Recombinant Antibody (SF98042-1-RR, Clone: 240427E12) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE Anti-GGGGS Linker Rabbit Recombinant Antibody (PE-98262, Clone: 242306C8). Cells were then stained with APC Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (APC-65569, Clone: OKT3). Cells were not fixed. Lymphocytes were gated.

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  Affinity and Kinetic Characterization of SF98042-1-RR

  Biolayer interferometry (BLl) kinetic assays of SF98042-1-RR against Human CD4 were performed. The affinity constant is 0.824 nM.

CD4 Recombinant Antibody for FC

Cat No. SF98042-1-RR

Clone No.240427E12

Host / Type

Rabbit / scFv

Reactivity

human, monkey, non-human primates

Applications

FC

Synonyms

CD4 molecule, CD4mut, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

SF98042-1-RR targets CD4 in FC applications and shows reactivity with human, monkey, non-human primates samples.

• Tested Reactivity: human, monkey, non-human primates
• Host / Type: Rabbit / scFv
• Class: Recombinant
• Type: Single-chain variable fragment antibody (scFv)
• Immunogen:

  CatNo: Eg0333

  Product name: Recombinant Human CD4 protein (His Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 26-396 aa of BC025782

  Sequence: KKVVLGKKGDTVELTCTASQKKSIQFHWKNSNQIKILGNQGSFLTKGPSKLNDRADSRRSLWDQGNFPLIIKNLKIEDSDTYICEVEDQKEEVQLLVFGLTANSDTHLLQGQSLTLTLESPPGSSPSVQCRSPRGKNIQGGKTLSVSQLELQDSGTWTCTVLQNQKKVEFKIDIVVLAFQKASSIVYKKEGEQVEFSFPLAFTVEKLTGSGELWWQAERASSSKSWITFDLKNKEVSVKRVTQDPKLQMGKKLPLHLTLPQALPQYAGSGNLTLALEAKTGKLHQEVNLVVMRATQLQKNLTCEVWGPTSPKLMLSLKLENKEAKVSKREKAVWVLNPEAGMWQCLLSDSGQVLLESNIKVLPTWSTPVQP

• Full Name: CD4 molecule
• Calculated Molecular Weight: 51 kDa
• GenBank Accession Number: BC025782
• Gene Symbol: CD4
• Gene ID (NCBI): 920
• ENSEMBL Gene ID: ENSG00000010610
• RRID: AB_3750144
• Conjugate: Unconjugated
• Source: Anti-Human CD4 scFv from clone 240427E12, consisting of VH-GGGGS linker-VL (N- to C-terminus), with a His-C tag at the C-terminus.
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: 50mM HEPES, 150mM NaCl, 5mM EDTA, 0.1% CHAPS, 10% Sucrose and 0.1% Proclin 300, pH 7.3.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment. 20ul sizes contain 0.1% BSA.

Background Information

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, the majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).