Tested Applications
| Positive IF/ICC detected in | HeLa cells |
| Positive FC detected in | mouse splenocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:400 |
| Flow Cytometry (FC) | FC : 0.5 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
65117-1-Ig targets CD44 in IF/ICC, FC applications and shows reactivity with Human, Mouse samples.
| Tested Reactivity | Human, Mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Dexamethasone-induced myeloid leukemia M1 cells Predict reactive species |
| Full Name | CD44 antigen |
| GenBank Accession Number | BC051388 |
| Gene Symbol | Cd44 |
| Gene ID (NCBI) | 12505 |
| RRID | AB_2918413 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P15379 |
| Storage Buffer | PBS with 0.09% sodium azide, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Stable for one year after shipment. |
Background Information
CD44 is a type I transmembrane glycoprotein expressed on embryonic stem cells and in various levels on other cell types including connective tissues and bone marrow. CD44 expression is also upregulated in subpopulations of cancer cells and is recognized as a molecular marker for cancer stem cells (PMID: 29747682). It is a cell-surface receptor that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands (PMID: 10694938). Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CD44 antibody 65117-1-Ig | Download protocol |
| IF protocol for CD44 antibody 65117-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Anti-Mouse CD44 (IM7) antibody (Cat# 65117-1-Ig) has 4 citations spanning four journals: Advanced Science, Journal of Controlled Release, International Journal of Biological Macromolecules, and Cancer Science. Research fields include Th17-mediated inflammation in Behçet's uveitis, ferroptosis-based cancer therapy, intestinal mucosal and systemic immunity, and colorectal cancer progression via CD8⁺ T cell exhaustion.
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) NETs-CD44-IL-17A Feedback Loop Drives Th17-Mediated Inflammation in Behçet's Uveitis | ||
J Control Release Unsaturated fatty acid-doped liposomes deliver piperine to deactivate defensive mechanism for ferroptosis in cancer therapy. | ||
Int J Biol Macromol The impact of novel oral adjuvant dendritic fibrous nano-silica coated with M cell homing peptide-modified chitosan on enhancing both the intestinal mucosal and systemic immunity. | ||
Cancer Sci INHBB Promotes Colorectal Cancer Progression by Inducing CD8(+) T Cell Exhaustion via the ACVR2B-SMAD Signaling Axis. |
