CoraLite® Plus 488 Anti-Mouse CD44 (IM7)

CD44 Monoclonal Antibody for FC, IF

Host / Isotype

Rat / IgG2b, kappa

Reactivity

Human, Mouse

Applications

IF, FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

IM7

Cat no : CL488-65117

Synonyms

AU023126, AW121933, AW146109, Cd44, CD44 antigen, HERMES, Ly 24, Pgp 1

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Validation Data Gallery

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  IF Staining of mouse splenocytes using CL488-65117

  Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 488 CD44 antibody (CL488-65117, Clone: IM7 ) at dilution of 1:100.

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  FC experiment of mouse splenocytes using CL488-65117

  1X10^6 C57BL/6 mouse splenocytes were surface stained with 5 ul CoraLite® Plus 647 Anti-Mouse CD3 (17A2) (CL647-65077, Clone: 17A2) and CoraLite® Plus 488 conjugated Rat IgG2b (left) or 5 ul CoraLite® Plus 488 Anti-Mouse CD44 (CL488-65117, Clone: IM7) (right). Cells were not fixed.

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Tested Applications

• Positive IF detected in: mouse splenocytes
• Positive FC detected in: mouse splenocytes

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-65117 targets CD44 in IF, FC applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Host / Isotype: Rat / IgG2b, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Dexamethasone-induced myeloid leukemia M1 cells
• Full Name: CD44 antigen
• GenBank Accession Number: BC051388
• Gene Symbol: Cd44
• Gene ID (NCBI): 12505
• RRID: AB_2883189
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD44 is a type I transmembrane glycoprotein expressed on embryonic stem cells and in various levels on other cell types including connective tissues and bone marrow. CD44 expression is also upregulated in subpopulations of cancer cells and is recognized as a molecular marker for cancer stem cells (PMID: 29747682). It is a cell-surface receptor that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands (PMID: 10694938). Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis.