CoraLite®594 Anti-Mouse CD44 (IM7)

CD44 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2b

Reactivity

Human, Mouse

Applications

FC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

IM7

Cat no : CL594-65117

Synonyms

AU023126, AW121933, AW146109, Cd44, CD44 antigen, HERMES, Ly 24, Pgp 1



Tested Applications

Positive FC detected inmouse splenocytes
For other applications, we recommend the unconjugated version of this antibody, 65117-1-Ig

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Product Information

CL594-65117 targets CD44 in FC applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Rat / IgG2b
Class Monoclonal
Type Antibody
Immunogen Dexamethasone-induced myeloid leukemia M1 cells
Full Name CD44 antigen
GenBank accession numberBC051388
Gene symbol Cd44
Gene ID (NCBI) 12505
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths593 nm/614 nm
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light.

Background Information

CD44 is a type I transmembrane glycoprotein expressed on embryonic stem cells and in various levels on other cell types including connective tissues and bone marrow. CD44 expression is also upregulated in subpopulations of cancer cells and is recognized as a molecular marker for cancer stem cells (PMID: 29747682). It is a cell-surface receptor that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands (PMID: 10694938). Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis.

Protocols

Product Specific Protocols
FC protocol for CL594 CD44 antibody CL594-65117Download protocol
Standard Protocols
Click here to view our Standard Protocols