|Positive FC detected in||human PBMCs|
|This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is N/A per 10^6 cells in a 100 µl suspension or N/A per 100 µl of whole blood.|
|Sample-dependent, check data in validation data gallery|
FITC-65063 targets CD44 in FC applications and shows reactivity with Human samples.
|Host / Isotype||Mouse / IgG2a, kappa|
|Immunogen||Purified T cells from human lymph nodes|
|Full Name||CD44 molecule (Indian blood group)|
|Calculated molecular weight||742 aa, 82 kDa|
|GenBank accession number||BC004372|
|Gene ID (NCBI)||960|
|Conjugate||FITC Fluorescent Dye|
|Excitation/Emission maxima wavelengths||494 nm / 520 nm|
|Purification Method||Affinity purification|
|Storage Buffer||PBS with 0.09% sodium azide and 0.5% BSA.|
|Storage Conditions||Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.|
CD44 is a type I transmembrane glycoprotein expressed on embryonic stem cells and in various levels on other cell types including connective tissues and bone marrow. CD44 expression is also upregulated in subpopulations of cancer cells and is recognized as a molecular marker for cancer stem cells (PMID: 29747682). It is a cell-surface receptor that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands (PMID: 10694938). Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis.