Anti-Human CD45 (HI30)

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  IF Staining of Jurkat using 65109-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD45 antibody (65109-1-Ig, Clone: HI30 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of human PBMCs using 65109-1-Ig

  1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD45 (65109-1-Ig, Clone:HI30) or Mouse IgG1 Isotype Control and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:500. Cells were not fixed. Lymphocytes were gated.

CD45 Monoclonal Antibody for IF/ICC, FC

Cat No. 65109-1-Ig

Clone No.HI30

Publications(2)

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

IF/ICC, FC

Synonyms

EC:3.1.3.48, GP180, HI30, L CA, LCA

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Tested Applications

• Positive IF/ICC detected in: Jurkat cells
• Positive FC detected in: human PBMCs

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000
• Flow Cytometry (FC): FC : 0.2 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below
• FC: See 1 publications below

Product Information

65109-1-Ig targets CD45 in IF/ICC, FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Human peripheral blood leucocytes
• Full Name: protein tyrosine phosphatase, receptor type, C
• GenBank Accession Number: BC014239
• Gene Symbol: CD45
• Gene ID (NCBI): 5788
• ENSEMBL Gene ID: ENSG00000081237
• RRID: AB_2918407
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: P08575
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).

Publications

Species	Application	Title
human	FC	Front Cell Dev Biol; Nrf2 Activation Is Involved in Cyclic Mechanical Stress-Stimulated Osteogenic Differentiation in Periodontal Ligament Stem Cells via PI3K/Akt Signaling and HO1-SOD2 Interaction.; Authors - Xun Xi
human	IF	Cell Discov; Integration of 3D bioprinting and multi-algorithm machine learning identified glioma susceptibilities and microenvironment characteristics; Authors - Min Tang