Anti-Human CD45 (HI30)

CD45 Monoclonal Antibody for FC, IF

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human and More (1)

Applications

IF, FC

Conjugate

Unconjugated

Publications

1

CloneNo.

HI30

Cat no : 65109-1-Ig

Synonyms

B220, CD45, CD45R, GP180, HI30, L CA, LCA, Leukocyte common antigen, LY5, PTPRC, RPTPC, T200

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Validation Data Gallery

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  IF Staining of Jurkat using 65109-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD45 antibody (65109-1-Ig, Clone: HI30 ) at dilution of 1:5000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of Jurkat using 65109-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD45 antibody (65109-1-Ig, Clone: HI30 ) at dilution of 1:5000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of human peripheral blood lymphocytes using 65109-1-Ig

  1X10^6 human peripheral blood lymphocytes were surface stained with 0.5 ug Anti-Human CD45 (65109-1-Ig, Clone: HI30) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), or stained with 0.5 ug isotype control antibody and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (black). Cells were not fixed.

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Tested Applications

• Positive IF detected in: Jurkat cells
• Positive FC detected in: human peripheral blood lymphocytes

Recommended dilution

• Immunofluorescence (IF): IF : 1:2500-1:10000

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Published Applications

• FC: See 1 publications below

Product Information

65109-1-Ig targets CD45 in IF, FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Human peripheral blood leucocytes
• Full Name: protein tyrosine phosphatase, receptor type, C
• GenBank accession number: BC014239
• Gene symbol: PTPRC
• Gene ID (NCBI): 5788
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: Phosphate based buffer with 0.09% sodium azide, pH 7.2.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).

Publications

Species	Application	Title
human	FC	Front Cell Dev Biol; Nrf2 Activation Is Involved in Cyclic Mechanical Stress-Stimulated Osteogenic Differentiation in Periodontal Ligament Stem Cells via PI3K/Akt Signaling and HO1-SOD2 Interaction.; Authors - Xun Xi