Anti-Human CD45 (HI30)
CD45 Monoclonal Antibody for FC, IF
Host / Isotype
Mouse / IgG1, kappa
Reactivity
Human and More (1)
Applications
IF, FC
Conjugate
Unconjugated
CloneNo.
HI30
Cat no : 65109-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive IF detected in | Jurkat cells |
Positive FC detected in | human peripheral blood lymphocytes |
Recommended dilution
Application | Dilution |
---|---|
Immunofluorescence (IF) | IF : 1:2500-1:10000 |
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Published Applications
FC | See 1 publications below |
Product Information
65109-1-Ig targets CD45 in IF, FC applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Cited Reactivity | human |
Host / Isotype | Mouse / IgG1, kappa |
Class | Monoclonal |
Type | Antibody |
Immunogen | Human peripheral blood leucocytes |
Full Name | protein tyrosine phosphatase, receptor type, C |
GenBank accession number | BC014239 |
Gene symbol | PTPRC |
Gene ID (NCBI) | 5788 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Affinity purification |
Storage Buffer | Phosphate based buffer with 0.09% sodium azide, pH 7.2. |
Storage Conditions | Store at 2-8°C. Stable for one year after shipment. |
Background Information
CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).
Protocols
Product Specific Protocols | |
---|---|
IF protocol for CD45 antibody 65109-1-Ig | Download protocol |
FC protocol for CD45 antibody 65109-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Front Cell Dev Biol Nrf2 Activation Is Involved in Cyclic Mechanical Stress-Stimulated Osteogenic Differentiation in Periodontal Ligament Stem Cells via PI3K/Akt Signaling and HO1-SOD2 Interaction. |