CD45 Recombinant antibody
CD45 Recombinant Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, Rat, Mouse and More (1)
Applications
WB, IHC, IF, FC, ELISA
Conjugate
Unconjugated
CloneNo.
6O19
Cat no : 80297-1-RR
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Daudi cells, Raji tissue, Ramos tissue |
| Positive IHC detected in | mouse colon tissue, human appendicitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | mouse colon tissue, mouse spleen tissue, Jurkat cells |
| Positive FC detected in | Raji cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunohistochemistry (IHC) | IHC : 1:4000-1:16000 |
| Immunofluorescence (IF) | IF : 1:125-1:500 |
| Flow Cytometry (FC) | FC : 0.15 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IF | See 1 publications below |
Product Information
80297-1-RR targets CD45 in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human, Rat, Mouse samples.
| Tested Reactivity | Human, Rat, Mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | CD45 fusion protein Ag13712 |
| Full Name | protein tyrosine phosphatase, receptor type, C |
| Calculated Molecular Weight | 1304 aa, 147 kDa |
| Observed Molecular Weight | 220-240 kDa |
| GenBank Accession Number | BC014239 |
| Gene Symbol | PTPRC |
| Gene ID (NCBI) | 5788 |
| RRID | AB_2918884 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD45, also known as leukocyte common antigen (LCA), B220, and T200, is a 180-240 kD single chain type I membrane glycoprotein. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 is one of the best negative selection markers for characterizing and/or isolating human mesenchymal stem cells from bone marrow and other sources. Along with the positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CD45 antibody 80297-1-RR | Download protocol |
| IHC protocol for CD45 antibody 80297-1-RR | Download protocol |
| IF protocol for CD45 antibody 80297-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Commun Biol An integrated map of fibroblastic populations in human colon mucosa and cancer tissues |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Kenzo (Verified Customer) (08-14-2023) | This antibody works well to immunolabel hematopoietic cells in mouse lymph nodes.
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