CD45 Recombinant antibody
CD45 Recombinant Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, Rat, Mouse and More (1)
Applications
WB, IHC, IF, FC, ELISA
Conjugate
Unconjugated
CloneNo.
6O19
Cat no : 80297-1-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Raji cells, THP-1 cells, Jurkat cells, K-562 cells, Ramos cells, Daudi cells |
Positive IHC detected in | human appendicitis tissue, human tonsillitis tissue, rat thymus tissue, mouse spleen tissue, mouse colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | Jurkat cells |
Positive FC detected in | Raji cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
IF | See 1 publications below |
Product Information
80297-1-RR targets CD45 in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human, Rat, Mouse samples.
Tested Reactivity | Human, Rat, Mouse |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | CD45 fusion protein Ag13712 |
Full Name | protein tyrosine phosphatase, receptor type, C |
Calculated molecular weight | 1304 aa, 147 kDa |
Observed molecular weight | 220-240 kDa |
GenBank accession number | BC014239 |
Gene symbol | PTPRC |
Gene ID (NCBI) | 5788 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CD45, also known as leukocyte common antigen (LCA), B220, and T200, is a 180-240 kD single chain type I membrane glycoprotein. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 is one of the best negative selection markers for characterizing and/or isolating human mesenchymal stem cells from bone marrow and other sources. Along with the positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for CD45 antibody 80297-1-RR | Download protocol |
IHC protocol for CD45 antibody 80297-1-RR | Download protocol |
IF protocol for CD45 antibody 80297-1-RR | Download protocol |
FC protocol for CD45 antibody 80297-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Commun Biol An integrated map of fibroblastic populations in human colon mucosa and cancer tissues |