CD45 Recombinant antibody

CD45 Recombinant Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Rat, Mouse and More (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

6O19

Cat no : 80297-1-RR

Synonyms

B220, CD45, CD45R, GP180, L CA, LCA, Leukocyte common antigen, LY5, PTPRC, RPTPC, T200

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Validation Data Gallery

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  WB analysis using 80297-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80297-1-RR (CD45 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of THP-1 using 80297-1-RR

  THP-1 cells were subjected to SDS PAGE followed by western blot with 80297-1-RR (CD45 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 80297-1-RR

  Jurkat cells were subjected to SDS PAGE followed by western blot with 80297-1-RR (CD45 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 80297-1-RR

  K-562 cells were subjected to SDS PAGE followed by western blot with 80297-1-RR (CD45 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human appendicitis using 80297-1-RR

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 80297-1-RR (CD45 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendicitis using 80297-1-RR

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 80297-1-RR (CD45 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 80297-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 80297-1-RR (CD45 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 80297-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 80297-1-RR (CD45 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 80297-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 80297-1-RR (CD45 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 80297-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 80297-1-RR (CD45 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 80297-1-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 80297-1-RR (CD45 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 80297-1-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 80297-1-RR (CD45 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat thymus using 80297-1-RR

  Immunohistochemical analysis of paraffin-embedded rat thymus tissue slide using 80297-1-RR (CD45 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat thymus using 80297-1-RR

  Immunohistochemical analysis of paraffin-embedded rat thymus tissue slide using 80297-1-RR (CD45 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of Jurkat using 80297-1-RR

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD45 antibody (80297-1-RR, Clone: 6O19 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of Raji using 80297-1-RR

  1X10^6 Raji cells were intracellularly stained with 0.15 ug Anti-Human CD45 (80297-1-RR, Clone: 6O19) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.15 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: Raji cells, THP-1 cells, Jurkat cells, K-562 cells, Ramos cells, Daudi cells
• Positive IHC detected in: human appendicitis tissue, human tonsillitis tissue, rat thymus tissue, mouse spleen tissue, mouse colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: Jurkat cells
• Positive FC detected in: Raji cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• IF: See 1 publications below

Product Information

80297-1-RR targets CD45 in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human, Rat, Mouse samples.

• Tested Reactivity: Human, Rat, Mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CD45 fusion protein Ag13712
• Full Name: protein tyrosine phosphatase, receptor type, C
• Calculated molecular weight: 1304 aa, 147 kDa
• Observed molecular weight: 220-240 kDa
• GenBank accession number: BC014239
• Gene symbol: PTPRC
• Gene ID (NCBI): 5788
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CD45, also known as leukocyte common antigen (LCA), B220, and T200, is a 180-240 kD single chain type I membrane glycoprotein. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 is one of the best negative selection markers for characterizing and/or isolating human mesenchymal stem cells from bone marrow and other sources. Along with the positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification.

Publications

Species	Application	Title
human	IF	Commun Biol; An integrated map of fibroblastic populations in human colon mucosa and cancer tissues; Authors - Siying Li

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C