CoraLite®555-conjugated CD45 Recombinant antibody

CD45 Recombinant Antibody for FC (Intra), IF

Host / Isotype

Rabbit / IgG

Reactivity

Human, Rat, Mouse

Applications

IF, FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

6O19

Reviews (1)

Cat no : CL555-80297

Synonyms

B220, CD45, CD45R, GP180, L CA, LCA, Leukocyte common antigen, LY5, PTPRC, RPTPC, T200

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Validation Data Gallery

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  IF Staining of mouse spleen using CL555-80297

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using CoraLite®555 CD45 antibody (CL555-80297, Clone: 6O19 ) at dilution of 1:200.

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  IF Staining of mouse spleen using CL555-80297

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using CoraLite®555 CD45 antibody (CL555-80297, Clone: 6O19 ) at dilution of 1:200.

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  FC experiment of Raji using CL555-80297

  1X10^6 Raji cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human CD45 (CL555-80297, Clone:6O19) (red), or 0.4 ug x. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF detected in: mouse spleen tissue
• Positive FC detected in: Raji cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL555-80297 targets CD45 in IF, FC (Intra) applications and shows reactivity with Human, Rat, Mouse samples.

• Tested Reactivity: Human, Rat, Mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CD45 fusion protein Ag13712
• Full Name: protein tyrosine phosphatase, receptor type, C
• Calculated Molecular Weight: 1304 aa, 147 kDa
• Observed Molecular Weight: 220-240 kDa
• GenBank Accession Number: BC014239
• Gene Symbol: PTPRC
• Gene ID (NCBI): 5788
• Conjugate: CoraLite®555 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 557 nm / 570 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD45, also known as leukocyte common antigen (LCA), B220, and T200, is a 180-240 kD single chain type I membrane glycoprotein. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 is one of the best negative selection markers for characterizing and/or isolating human mesenchymal stem cells from bone marrow and other sources. Along with the positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification.

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Sonja (Verified Customer) (01-27-2023)

The antibody is perfect. No background and very strong specific signal!

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: FFPE Tonsil

CL555 CD45 Antibody Immunofluorescence validation (1:50 dilution) in FFPE Tonsil (Cat no:CL555-80297)