CoraLite® Plus 555 Anti-Rat CD45 (OX1)

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  FC experiment of rat splenocytes using CL555-65228

  1X10^6 rat splenocytes were surface stained with 0.5 ug CoraLite® Plus 555 Anti-Rat CD45 (CL555-65228, Clone:OX1) (red), or 0.5 ug Isotype Control. Cells were not fixed.

CD45 Monoclonal Antibody for FC

Cat No. CL555-65228

Clone No.OX1

Host / Isotype

Mouse / IgG1, kappa

Reactivity

rat

Applications

FC

Synonyms

Lca, Ptprc, L CA, OX1, RT7

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Tested Applications

• Positive FC detected in: rat splenocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.5 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL555-65228 targets CD45 in FC applications and shows reactivity with rat samples.

• Tested Reactivity: rat
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  N/A

• Full Name: protein tyrosine phosphatase, receptor type, C
• Calculated Molecular Weight: 143 kDa
• GenBank Accession Number: XM_006249910
• Gene Symbol: CD45
• Gene ID (NCBI): 24699
• RRID: AB_3084602
• Conjugate: CoraLite® Plus 555 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 554 nm / 570 nm
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: P04157
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).