Tested Applications
| Positive FC detected in | Wistar rat splenocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 1 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
FITC-65228 targets CD45 in FC applications and shows reactivity with rat samples.
| Tested Reactivity | rat |
| Cited Reactivity | rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
N/A Predict reactive species |
| Full Name | protein tyrosine phosphatase, receptor type, C |
| Calculated Molecular Weight | 143 kDa |
| GenBank Accession Number | XM_006249910 |
| Gene Symbol | CD45 |
| Gene ID (NCBI) | 24699 |
| RRID | AB_2924145 |
| Conjugate | FITC Plus Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 495 nm / 524 nm |
| Excitation Laser | Blue laser (488 nm) |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | P04157 |
| Storage Buffer | PBS with 0.09% sodium azide, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for FITC Plus CD45 antibody FITC-65228 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
FITC-65228 is an Anti-Rat CD45 (OX1) monoclonal antibody. It has 1 publication citation, appearing in the journal Molecules (2024). The cited study investigated persistent photoconductivity of gallium nitride semiconductor and differentiation of primary neural stem cells, placing the research in the fields of neuroscience/stem cell biology and materials science. The antibody was used in flow cytometry (FC) on rat samples.
| Species | Application | Title |
|---|---|---|
Molecules Investigation of Persistent Photoconductivity of Gallium Nitride Semiconductor and Differentiation of Primary Neural Stem Cells |
