Anti-Mouse CD45R (B220) (RA3-6B2)

CD45R (B220) Monoclonal Antibody for IF/ICC, IF-P, FC
Cat No. 65139-1-Ig
Clone No.RA3-6B2

Host / Isotype

Rat / IgG2a, kappa

Reactivity

mouse

Applications

IF/ICC, IF-P, FC

B220, CD45R, Cd45, Ptprc, EC:3.1.3.48

Formulation:  PBS and Azide
PBS and Azide
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive IF-P detected inmouse spleen tissue
Positive IF/ICC detected inmouse splenocytes
Positive FC detected inmouse splenocytes

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC)FC : 0.5 ug per 10^6 cells in 100 μl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

65139-1-Ig targets CD45R (B220) in IF/ICC, IF-P, FC applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Cited Reactivitymouse
Host / Isotype Rat / IgG2a, kappa
Class Monoclonal
Type Antibody
Immunogen Abelson murine leukemia virus-induced pre-B tumor cells Predict reactive species
Full Name protein tyrosine phosphatase, receptor type, C
GenBank Accession NumberBC028512
Gene Symbol CD45
Gene ID (NCBI) 19264
RRIDAB_2918426
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP06800
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45R (B220) is an isoform of CD45, expressed on murine B-lineage cells (except for plasma cells) and on a small sub-population of T cells (PMID: 6975314; 15712182). It is commonly used as a pan B-cell marker in mice. CD45R is also expressed on activated T cells and NK cell subsets (PMID: 7514808; 17923504). The RA3-6B2 clone reacts with human and mouse CD45R.

Protocols

Product Specific Protocols
IF protocol for CD45R (B220) antibody 65139-1-IgDownload protocol
FC protocol for CD45R (B220) antibody 65139-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Thorac Cancer

Experimental model of micronodular thymic neoplasm with lymphoid stroma

Authors - Riko Niwa
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