APC Anti-Mouse CD45R (B220) (RA3-6B2)

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  FC experiment of mouse splenocytes using APC-65139

  1X10^6 mouse splenocytes were surface co-stained with CoraLite® Plus 488 Anti-Mouse CD3 (17A2) and 0.1 ug APC Anti-Mouse CD45R (B220) (APC-65139, Clone:RA3-6B2) or 0.1 ug APC-Rat IgG2a isotype control. Cells were not fixed.

CD45R (B220) Monoclonal Antibody for FC

Cat No. APC-65139

Clone No.RA3-6B2

Host / Isotype

Rat / IgG2a, kappa

Reactivity

mouse

Applications

FC

Synonyms

B220, CD45R, Cd45, Ptprc, EC:3.1.3.48

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.1 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

APC-65139 targets CD45R (B220) in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rat / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Abelson murine leukemia virus-induced pre-B tumor cells

• Full Name: protein tyrosine phosphatase, receptor type, C
• GenBank Accession Number: BC028512
• Gene Symbol: CD45
• Gene ID (NCBI): 19264
• RRID: AB_2883020
• Conjugate: APC Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 650 nm / 660 nm
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: P06800
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45R (B220) is an isoform of CD45, expressed on murine B-lineage cells (except for plasma cells) and on a small sub-population of T cells (PMID: 6975314; 15712182). It is commonly used as a pan B-cell marker in mice. CD45R is also expressed on activated T cells and NK cell subsets (PMID: 7514808; 17923504). The RA3-6B2 clone reacts with human and mouse CD45R.