Tested Applications
| Positive FC detected in | mouse splenocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.5 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IF | See 1 publications below |
Product Information
CL555-65139 targets CD45R (B220) in IF, FC applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Abelson murine leukemia virus-induced pre-B tumor cells Predict reactive species |
| Full Name | protein tyrosine phosphatase, receptor type, C |
| GenBank Accession Number | BC028512 |
| Gene Symbol | CD45 |
| Gene ID (NCBI) | 19264 |
| RRID | AB_3084561 |
| Conjugate | CoraLite® Plus 555 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 554 nm / 570 nm |
| Excitation Laser | Green Laser (532 nm), Yellow-Green Laser (561 nm) |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | P06800 |
| Storage Buffer | PBS with 0.09% sodium azide, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45R (B220) is an isoform of CD45, expressed on murine B-lineage cells (except for plasma cells) and on a small sub-population of T cells (PMID: 6975314; 15712182). It is commonly used as a pan B-cell marker in mice. CD45R is also expressed on activated T cells and NK cell subsets (PMID: 7514808; 17923504). The RA3-6B2 clone reacts with human and mouse CD45R.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 555 CD45R (B220) antibody CL555-65139 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
