Anti-Human CD45RB/RAB Rabbit Recombinant Antibody

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• Anti-Human CD45RB/RAB Rabbit Recombinant Antibody (98531-5-RR)

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  FC experiment of human peripheral blood leukocyte using 98531-5-RR

  1x10^6 human peripheral blood leukocyte were surface stained with 0.25 ug Anti-Human CD45RB/RAB Rabbit RecAb (98531-5-RR, Clone:242918F3), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L).Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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  FC experiment of human peripheral blood leukocyte using 98531-5-RR

  1x10^6 human peripheral blood leukocyte were surface stained with 0.25 ug Anti-Human PTPRC/CD45-RB Rabbit RecAb (98531-5-RR, Clone:242918F3) (red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue) and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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  FC experiment of Transfected CHO using 98531-5-RR

  1x10^6 untransfected or transfected CHO cells were surface stained with 0.25 ug Anti-Human CD45RB/RAB Rabbit RecAb (98531-5-RR, Clone: 242918F3) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

CD45RB/RAB Uni-rAb^® Recombinant Antibody for FC

Cat No. 98531-5-RR

Clone No.242918F3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

CD45, CD45RAB, CD45RB, EC:3.1.3.48, GP180

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Tested Applications

• Positive FC detected in: human peripheral blood leukocyte, transfected CHO cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98531-5-RR targets CD45RB/RAB in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: protein tyrosine phosphatase, receptor type, C
• Calculated Molecular Weight: 147 kDa
• GenBank Accession Number: NM_002838.4
• Gene Symbol: CD45
• Gene ID (NCBI): 5788
• ENSEMBL Gene ID: ENSG00000081237
• RRID: AB_3746158
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P08575-3
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

CD45RB and CD45RAB are specific isoforms of CD45, a receptor-type protein tyrosine phosphatase (PTP) found on the surface of all nucleated hematopoietic cells, except mature erythrocytes and platelets. They serve as essential phenotypic markers to distinguish naïve T lymphocytes from antigen-experienced memory/effector T cells. CD45RB and CD45RAB designations refer to specific isoforms of CD45 generated through alternative splicing of three variable exons (4, 5, and 6) in the PTPRC gene. These exons encode extracellular domains A, B, and C, respectively, which contain O-linked glycosylation sites that affect the molecule's size, charge, and ligand-binding properties. This antibody can detect CD45RB (contains exons B only) and CD45RAB (contains both exons A and B), but not CD45RABC (contain all three exons) or CD45RBC (contain exons B and C).