Anti-Human CD46 Rabbit Recombinant Antibody

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  FC experiment of human PBMCs using 98240-1-RR

  1x10^6 human PBMCs were surface stained with 0.125 ug Anti-Human CD46 Rabbit Recombinant Antibody (98240-1-RR, Clone: 241764C9) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD19. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using 98240-1-RR

  1x10^6 human PBMCs were surface stained with 0.125 ug Anti-Human CD46 Rabbit Recombinant Antibody (98240-1-RR, Clone: 241764C9) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

CD46 Uni-rAb^TM Recombinant Antibody for FC

Cat No. 98240-1-RR

Clone No.241764C9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

MCP, Membrane cofactor protein, MIC10, TLX, TRA2.10

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 0.125 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98240-1-RR targets CD46 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CD46 fusion protein Eg1770
• Full Name: CD46 molecule, complement regulatory protein
• Calculated Molecular Weight: 42kDa
• GenBank Accession Number: NM_172351.3
• Gene Symbol: CD46
• Gene ID (NCBI): 4179
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P15529-11
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

CD46, also known as Membrane cofactor protein (MCP) or MIC10, is a ubiquitously expressed type type-I transmembrane glycoprotein that acts as a cofactor for complement factor I to mediate inactivation of C3b and C4b deposited on host cells. CD46 is a receptor for a large number of pathogens, including measles virus and adenovirus (PMID: 20941397). CD46 plays a role in linking innate and adaptive immune responses. It may be involved in the fusion of the spermatozoa with the oocyte during fertilization. Mutations of the CD46 gene have been associated with some diseases including hemolytic uremic syndrome (PMID: 26054645). CD46 is expressed on most cells as four isoforms that are derived via alternative splicing (PMID: 15324743).