Tested Applications
| Positive FC detected in | human PBMCs |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 5 ul per 10^6 cells in a 100 µl suspension |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul / test per 10^6 cells in a 100 µl suspension or 5 ul / test per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CP-98240 targets CD46 in FC applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | CD46 fusion protein Eg1770 Predict reactive species |
| Full Name | CD46 molecule, complement regulatory protein |
| Calculated Molecular Weight | 42kDa |
| GenBank Accession Number | NM_172351.3 |
| Gene Symbol | CD46 |
| Gene ID (NCBI) | 4179 |
| Conjugate | PerCP Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 482 nm / 677 nm |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | P15529-11 |
| Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD46, also known as Membrane cofactor protein (MCP) or MIC10, is a ubiquitously expressed type type-I transmembrane glycoprotein that acts as a cofactor for complement factor I to mediate inactivation of C3b and C4b deposited on host cells. CD46 is a receptor for a large number of pathogens, including measles virus and adenovirus (PMID: 20941397). CD46 plays a role in linking innate and adaptive immune responses. It may be involved in the fusion of the spermatozoa with the oocyte during fertilization. Mutations of the CD46 gene have been associated with some diseases including hemolytic uremic syndrome (PMID: 26054645). CD46 is expressed on most cells as four isoforms that are derived via alternative splicing (PMID: 15324743).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for PerCP CD46 antibody CP-98240 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
