CD57 Monoclonal antibody

CD57 Monoclonal Antibody for FC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse

Applications

WB, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1F4A11

Cat no : 66711-1-Ig

Synonyms

B3GAT1, CD57, GlcAT P, GLCATP, GlcUAT P, Glucuronosyltransferase P, HNK 1, HNK1, LEU7, NK 1

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Validation Data Gallery

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  WB analysis of mouse brain using 66711-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66711-1-Ig (CD57 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 66711-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66711-1-Ig (CD57 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  FC experiment of K-562 using 66711-1-Ig

  1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human CD57 (66711-1-Ig, Clone:1F4A11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: K-562 cells, mouse brain tissue, Jurkat cells, PC-3 cells, SH-SY5Y cells, U -251 cells
• Positive FC detected in: K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000

Sample-dependent, check data in validation data gallery

Product Information

66711-1-Ig targets CD57 in WB, FC, ELISA applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD57 fusion protein Ag13593
• Full Name: beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P)
• Calculated molecular weight: 38 kDa
• Observed molecular weight: 38 and 100 kDa
• GenBank accession number: BC010466
• Gene symbol: B3GAT1
• Gene ID (NCBI): 27087
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

B3GAT1 is associated with glycosaminoglycan biosynthesis. It plays a key role in neurodevelopment. The calculated molecular weight of B3GAT1 is 38 kDa and 66711-1-Ig antibody detectes 100 kDa form in SDS-PAGE which might as a result of homodimer or glycosylation.

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C