B3GAT1 Monoclonal antibody

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  WB analysis using 66711-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66711-1-Ig (B3GAT1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 66711-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66711-1-Ig (B3GAT1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  FC experiment of K-562 using 66711-1-Ig

  1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human CD57 (66711-1-Ig, Clone:1F4A11) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

B3GAT1 Monoclonal Antibody for WB, FC (Intra), ELISA

Cat No. 66711-1-Ig

Clone No.1F4A11

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Synonyms

GlcAT-P, GLCATP, Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1, EC:2.4.1.135, Beta-1,3-glucuronyltransferase 1

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Tested Applications

• Positive WB detected in: K-562 cells, mouse brain tissue, Jurkat cells, PC-3 cells, SH-SY5Y cells, U-251 cells
• Positive FC (Intra) detected in: K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66711-1-Ig targets B3GAT1 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: B3GAT1 fusion protein Ag13593
• Full Name: beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P)
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 38 kDa, 100 kDa
• GenBank Accession Number: BC010466
• Gene Symbol: B3GAT1
• Gene ID (NCBI): 27087
• RRID: AB_2882062
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9P2W7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

B3GAT1 is associated with glycosaminoglycan biosynthesis. It plays a key role in neurodevelopment. The calculated molecular weight of B3GAT1 is 38 kDa and 66711-1-Ig antibody detectes 100 kDa form in SDS-PAGE which might as a result of homodimer or glycosylation.