Anti-Human CD62L (DREG56)

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  FC experiment of human PBMCs using 65167-1-Ig

  1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD62L (65167-1-Ig, Clone:DREG56) or Mouse IgG1 Isotype Control and then CoraLite®647-conjugated F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8). Cells were then stained with CL488 Anti-Human CD3 (CL488-65151). Cells were not fixed. Lymphocytes were gated.

CD62L Monoclonal Antibody for FC

Cat No. 65167-1-Ig

Clone No.DREG56

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

FC

Synonyms

L-selectin, L selectin, hLHRc, gp90-MEL, gp90 MEL

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65167-1-Ig targets CD62L in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: PMA-activated human peripheral blood leukocytes
• Full Name: selectin L
• Calculated Molecular Weight: 42 kDa
• GenBank Accession Number: BC020758
• Gene Symbol: CD62L
• Gene ID (NCBI): 6402
• ENSEMBL Gene ID: ENSG00000188404
• RRID: AB_2918444
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Purified by protein-A affinity chromatography
• UNIPROT ID: P14151
• Storage Buffer: PBS with 0.09% sodium azide , pH 7.3
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD62L, also known as L-selectin or SELL, is a member of the selectin family of adhesion molecules that also include CD62E (E-selectin) and CD62P (P-selectin) (PMID: 2663882, 2473156, 1382078). CD62L is a highly glycosylated protein of 95-105 kDa on neutrophils and 74 kDa on lymphocytes (PMID: 1382078; 1694883, 1695155). CD62L is expressed on the surface of most leukocytes, including lymphocytes, neutrophils, monocytes, eosinophils, hematopoietic progenitor cells, and immature thymocytes (PMID: 1694883, 1688580). It mediates the binding of lymphocytes to high endothelial venules (HEV) of peripheral lymph nodes through interactions with a constitutively expressed ligand, and is also involved in lymphocyte, neutrophil, and monocyte attachment to endothelium at sites of inflammation (PMID: 1382078).