FITC Anti-Human CD62L (DREG56)
CD62L Monoclonal Antibody for FC
Host / Isotype
Mouse / Mouse IgG1
Reactivity
Human
Applications
FC
Conjugate
FITC Fluorescent Dye
CloneNo.
DREG56
Cat no : FITC-65167
Synonyms
Validation Data Gallery
Tested Applications
Positive FC detected in | Human peripheral blood lymphocytes |
Recommended dilution
Application | Dilution |
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This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 20 µl per 10^6 cells in a 100 µl suspension or 20 µl per 100 µl of whole blood. | |
Sample-dependent, check data in validation data gallery |
Product Information
FITC-65167 targets CD62L in FC applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Host / Isotype | Mouse / Mouse IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | PMA-activated human peripheral blood leukocytes |
Full Name | selectin L |
Calculated molecular weight | 42 kDa |
GenBank accession number | BC020758 |
Gene symbol | SELL |
Gene ID (NCBI) | 6402 |
Conjugate | FITC Fluorescent Dye |
Excitation/Emission maxima wavelengths | 494 nm / 520 nm |
Form | Liquid |
Purification Method | The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography. |
Storage Buffer | PBS with 0.1% sodium azide. |
Storage Conditions | Store at 2-8°C. Avoid exposure to light. |
Background Information
CD62L, also known as L-selectin or SELL, is a member of the selectin family of adhesion molecules that also include CD62E (E-selectin) and CD62P (P-selectin) (PMID: 2663882, 2473156, 1382078). CD62L is a highly glycosylated protein of 95-105 kDa on neutrophils and 74 kDa on lymphocytes (PMID: 1382078; 1694883, 1695155). CD62L is expressed on the surface of most leukocytes, including lymphocytes, neutrophils, monocytes, eosinophils, hematopoietic progenitor cells, and immature thymocytes (PMID: 1694883, 1688580). It mediates the binding of lymphocytes to high endothelial venules (HEV) of peripheral lymph nodes through interactions with a constitutively expressed ligand, and is also involved in lymphocyte, neutrophil, and monocyte attachment to endothelium at sites of inflammation (PMID: 1382078).
Protocols
Product Specific Protocols | |
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FC protocol for FITC CD62L antibody FITC-65167 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |