Biotin Anti-Mouse CD68 Rabbit Recombinant Antibody

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  FC experiment of mouse peritoneal macrophages using Biotin-98029

  1x10^6 mouse peritoneal macrophages were intracellularly stained with 0.25 ug Biotin Anti-Mouse CD68 Rabbit RecAb (Biotin-98029, Clone:230504G5), or unstained and Streptavidin-PE Conjugate (PE-PF00030). Cells were co-stained with APC Anti-Mouse CD11b (M1/70) (APC-65055, Clone: M1/70). Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

• 

  FC experiment of mouse peritoneal macrophages using Biotin-98029

  1x10^6 mouse peritoneal macrophages were intracellularly stained with 0.25 ug Biotin Anti-Mouse CD68 Rabbit RecAb (Biotin-98029, Clone:230504G5) (blue), or unstained(blue) and Streptavidin-PE Conjugate (PE-PF00030). Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

CD68 Uni-rAb^® Recombinant Antibody for FC (Intra), ELISA

Cat No. Biotin-98029

Clone No.230504G5

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC (Intra), ELISA

Synonyms

230504G5, CD68 antigen, gp110, Scard1

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Tested Applications

• Positive FC (Intra) detected in: mouse peritoneal macrophages

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-98029 targets CD68 in FC (Intra), ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg0787

  Product name: Recombinant mouse CD68 protein

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 21-291 aa of NM_001291058.1

  Sequence: DCPHKKAVTLLPSFTMTPTATESTASPTTSHRPTTTSHGNVTVHTSSGPTTVTHNPATTTSHGNATISHATVSPTTNGTATSPRSSTVGPHPGPPPPSPSPRSKGALGNYTWANGSQPCVQLQAQIQIRILYPIQGGRKAWGISVLNPNKTKVQGGCDGTHPHLSLSFPYGQLTFGFKQDLHQSPSTVYLDYMAVEYNVSFPQAAQWTFMAQNSSLRELQAPLGQSFCCGNASIVLSPAVHLDLLSLRLQAAQLPDKGHFGPCFSCNRDQS

• Full Name: CD68 antigen
• Calculated Molecular Weight: 35 kDa
• GenBank Accession Number: NM_001291058.1
• Gene Symbol: Cd68
• Gene ID (NCBI): 12514
• Conjugate: Biotin
• Excitation/Emission Maxima Wavelengths: -
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P31996-1
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Mouse CD68 (also known as macrosialin) is a type I transmembrane glycoprotein that is highly and specifically expressed by mouse tissue macrophages, and to a lesser extent by dendritic cells. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.