CoraLite® Plus 750 Anti-Mouse CD68 Rabbit Recombinant Antibody

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  FC experiment of mouse peritoneal macrophages using CL750-98029

  1x10^6 mouse peritoneal macrophages were intracellularly stained with CoraLite® Plus 488 Anti-Mouse CD11b and 0.25 ug CoraLite® Plus 750 Anti-Mouse CD68 Rabbit Recombinant Antibody (CL750-98029, Clone:230504G5), or Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

• 

  FC experiment of mouse peritoneal macrophages using CL750-98029

  1x10^6 mouse peritoneal macrophages were intracellularly stained with 0.25 ug CoraLite® Plus 750 Anti-Mouse CD68 Rabbit Recombinant Antibody (CL750-98029, Clone:230504G5)(red), or Isotype Control(blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

CD68 Uni-rAb^® Recombinant Antibody for FC (Intra)

Cat No. CL750-98029

Clone No.230504G5

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC (Intra)

Synonyms

230504G5, CD68 antigen, gp110, Scard1

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Tested Applications

• Positive FC (Intra) detected in: mouse peritoneal macrophages

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL750-98029 targets CD68 in FC (Intra) applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg0787

  Product name: Recombinant mouse CD68 protein

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 21-291 aa of NM_001291058.1

  Sequence: DCPHKKAVTLLPSFTMTPTATESTASPTTSHRPTTTSHGNVTVHTSSGPTTVTHNPATTTSHGNATISHATVSPTTNGTATSPRSSTVGPHPGPPPPSPSPRSKGALGNYTWANGSQPCVQLQAQIQIRILYPIQGGRKAWGISVLNPNKTKVQGGCDGTHPHLSLSFPYGQLTFGFKQDLHQSPSTVYLDYMAVEYNVSFPQAAQWTFMAQNSSLRELQAPLGQSFCCGNASIVLSPAVHLDLLSLRLQAAQLPDKGHFGPCFSCNRDQS

• Full Name: CD68 antigen
• Calculated Molecular Weight: 35 kDa
• GenBank Accession Number: NM_001291058.1
• Gene Symbol: Cd68
• Gene ID (NCBI): 12514
• RRID: AB_3673791
• Conjugate: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 755 nm / 780 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P31996-1
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Mouse CD68 (also known as macrosialin) is a type I transmembrane glycoprotein that is highly and specifically expressed by mouse tissue macrophages, and to a lesser extent by dendritic cells. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.