Biotin Anti-Human CD7 Rabbit Recombinant Antibody

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  FC experiment of human PBMCs using Biotin-98204

  1x10^6 human PBMCs were surface stained with 5 ul Biotin Anti-Human CD7 Rabbit RecAb (Biotin-98204, Clone: 242402H8) and CoraLite®488-conjugated streptavidin, or unstained. Cells were co-stained with APC Anti-Human CD4 (RPA-T4) (APC-65143, Clone: RPA-T4). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using Biotin-98204

  1x10^6 human PBMCs were surface stained with 5 ul Biotin Anti-Human CD7 Rabbit RecAb (Biotin-98204, Clone: 242402H8) and CoraLite®488-conjugated streptavidin (red), or unstained (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

CD7 Uni-rAb^TM Recombinant Antibody for FC

Cat No. Biotin-98204

Clone No.242402H8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

242402H8, CD7 molecule, GP40, LEU-9, T cell antigen CD7

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-98204 targets CD7 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: CD7 molecule
• Calculated Molecular Weight: 25 kDa
• GenBank Accession Number: NM_006137.7
• Gene Symbol: CD7
• Gene ID (NCBI): 924
• ENSEMBL Gene ID: ENSG00000173762
• Conjugate: Biotin
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P09564
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD7, or T-cell antigen 7 or GP40, is a transmembrane glycoprotein and a member of the immunoglobulin supergene family. CD7 is predominantly expressed on thymocytes and mature T cells, as well as natural killer (NK) cells, and it is one of the earliest antigens to appear on cells of the T-lymphocyte lineage. CD7 plays a significant role in T-cell and T-cell/B-cell interactions during early lymphoid development. It is involved in the regulation of cell signaling and biology, including T-cell activation, proliferation, and the expression of interleukin-2 receptor alpha (IL-2Rα). CD7 is not only a marker for T-cells but also has functional implications in immune cell interactions and adhesion. It can provide co-stimulatory signals and is associated with CD3 and CD45, participating in T-cell activation.