Tested Applications
| Positive FC detected in | mouse splenocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL594-98199 targets CD73 in FC applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg1389 Product name: Recombinant Mouse 5'-Nucleotidase/CD73 protein (rFc Tag) Source: mammalian cells-derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 29-551 aa of NM_011851.4 Sequence: WELTILHTNDVHSRLEQTSDDSTKCLNASLCVGGVARLFTKVQQIRKEEPNVLFLDAGDQYQGTIWFTVYKGLEVAHFMNILGYDAMALGNHEFDNGVEGLIDPLLRNVKFPILSANIKARGPLAHQISGLFLPSKVLSVGGEVVGIVGYTSKETPFLSNPGTNLVFEDEISALQPEVDKLKTLNVNKIIALGHSGFEMDKLIAQKVRGVDIVVGGHSNTFLYTGNPPSKEVPAGKYPFIVTADDGRQVPVVQAYAFGKYLGYLKVEFDDKGNVITSYGNPILLNSSIPEDATIKADINQWRIKLDNYSTQELGRTIVYLDGSTQTCRFRECNMGNLICDAMINNNLRHPDEMFWNHVSMCIVNGGGIRSPIDEKNNGTITWENLAAVLPFGGTFDLVQLKGSTLKKAFEHSVHRYGQSTGEFLQVGGIHVVYDINRKPWNRVVQLEVLCTKCRVPIYEPLEMDKVYKVTLPSYLANGGDGFQMIKDELLKHDSGDQDISVVSEYISKMKVVYPAVEGRIKFS Predict reactive species |
| Full Name | 5' nucleotidase, ecto |
| Calculated Molecular Weight | 64kDa |
| GenBank Accession Number | NM_011851.4 |
| Gene Symbol | Nt5e |
| Gene ID (NCBI) | 23959 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q61503 |
| Storage Buffer | PBS with 0.09% sodium azide, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD73 is a membrane-bound enzyme that catalyzes the extracellular hydrolysis of adenosine monophosphate (AMP) to produce adenosine. It is expressed on various immune cells, including T cells, B cells, dendritic cells, and regulatory T cells (Tregs). CD73 works in conjunction with CD39 to break down extracellular ATP into adenosine, creating an immunosuppressive environment that can inhibit the function of cytotoxic T cells and natural killer (NK) cells. This process is essential for immune tolerance and the resolution of inflammation. CD73 is also an important player in controlling tumor progression.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL594 CD73 antibody CL594-98199 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
