FC experiment of mouse splenocytes using CL647-98199
1x10^6 mouse splenocytes were surface stained with CoraLite® Plus 488 Anti-Mouse CD3, and 0.25 ug CoraLite® Plus 647 Anti-Mouse CD73 Rabbit RecAb (CL647-98199, Clone:241541G9) or CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9). Cells were not fixed.
1x10^6 mouse splenocytes were surface stained with CoraLite® Plus 488 Anti-Mouse CD3, and 0.25 ug CoraLite® Plus 647 Anti-Mouse CD73 Rabbit RecAb (CL647-98199, Clone:241541G9) or CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9). Cells were not fixed.
FC experiment of mouse splenocytes using CL647-98199
1x10^6 mouse splenocytes were surface stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse CD73 Rabbit RecAb (CL647-98199, Clone: 241541G9)(red) or CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9) (blue). Cells were not fixed.
1x10^6 mouse splenocytes were surface stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse CD73 Rabbit RecAb (CL647-98199, Clone: 241541G9)(red) or CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9) (blue). Cells were not fixed.
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This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
CL647-98199 targets CD73 in FC applications and shows reactivity with mouse samples.
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
Background Information
CD73, also known as ecto-5'-nucleotidase (5'-NT), is a 70-kDa, glycosyl-phosphatidylinositol-linked membrane-bound glycoprotein found in most tissues (PMID: 18404475; 20179192). CD73 is an ectoenzyme that catalyzes the dephosphorylation of AMP and other nucleoside monophosphates (PMID: 9553767)
Protocols
Product Specific Protocols
FC protocol for CL Plus 647 CD73 antibody CL647-98199
FC experiment of mouse splenocytes using CL647-98199
1x10^6 mouse splenocytes were surface stained with CoraLite® Plus 488 Anti-Mouse CD3, and 0.25 ug CoraLite® Plus 647 Anti-Mouse CD73 Rabbit RecAb (CL647-98199, Clone:241541G9) or CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9). Cells were not fixed.
FC experiment of mouse splenocytes using CL647-98199
1x10^6 mouse splenocytes were surface stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse CD73 Rabbit RecAb (CL647-98199, Clone: 241541G9)(red) or CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9) (blue). Cells were not fixed.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
CD73 Recombinant antibody
Catalog Number
CL647-98199
Citations
-
Dilutions
Applications
FC
Reactivity
mouse
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
