CoraLite® Plus 647 Anti-Human CD79a (HM57) Mouse IgG2a Recombinant Antibody

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  FC experiment of human PBMCs using CL647-65610

  1x10^6 human PBMCs were intracellularly stained with 5 ul CoraLite® Plus 647 Anti-Human CD79a (HM57) Mouse IgG2a RecAb (CL647-65610, Clone: HM57) or CoraLite® Plus 647 Mouse IgG2a Isotype Control (C1.18.4) (CL647-65208, Clone: C1.18.4). Cells were co-stained with CoraLite® Plus 488 Anti-Human CD19. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of human PBMCs using CL647-65610

  1x10^6 human PBMCs were intracellularly stained with 5 ul CoraLite® Plus 647 Anti-Human CD79a (HM57) Mouse IgG2a RecAb (CL647-65610, Clone: HM57) (red) or CoraLite® Plus 647 Mouse IgG2a Isotype Control (C1.18.4) (CL647-65208, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CD79a Uni-rAb^® Recombinant Antibody for FC (Intra)

Cat No. CL647-65610

Clone No.HM57

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC (Intra)

Synonyms

B-cell antigen receptor complex-associated protein alpha chain, MB 1, MB 1 membrane glycoprotein, MB1, MB-1 membrane glycoprotein

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Tested Applications

• Positive FC (Intra) detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 5 ul per 10^6 cells in a 100 µl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65610 targets CD79a in FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen:

  fusion protein

• Full Name: CD79a molecule, immunoglobulin-associated alpha
• GenBank Accession Number: BC113731
• Gene Symbol: CD79A
• Gene ID (NCBI): 973
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P11912
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD79a, also named as B-cell antigen receptor complex-associated protein alpha chain or MB-1 membrane glycoprotein, is a 226 amino acid protein, which contains one ITAM domain and one Ig-like C2-type (immunoglobulin-like) domain. CD79a is expressed in B cell and localizes in the cell membrane. CD79a is required in cooperation with CD79b for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. CD79a is also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells.