FcZero-rAb™ PE Anti-Human CD79a (HM57) Rabbit IgG Recombinant Antibody

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  FC experiment of human PBMCs using PE-FcA65610

  1x10^6 human PBMCs were intracellularly stained with FcZero-rAb™ APC Anti-Human CD19 (4G7), and 5 ul PE Anti-Human CD79a (HM57) Rabbit IgG RecAb (PE-FcA65610, Clone:HM57) or PE Rabbit IgG Isotype Control RecAb (PE-FcA98136, Clone: 240953C9). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CD79a FcZero-rAb^TM Recombinant Antibody for FC (Intra)

Cat No. PE-FcA65610

Clone No.HM57

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Synonyms

MB-1 membrane glycoprotein, MB1, MB 1 membrane glycoprotein, MB 1, HM57

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Tested Applications

• Positive FC (Intra) detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

PE-FcA65610 targets CD79a in FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Synthetic peptide corresponding to 202-216 amino acid sequence of human CD79a
• Full Name: CD79a molecule, immunoglobulin-associated alpha
• GenBank Accession Number: BC113731
• Gene Symbol: CD79A
• Gene ID (NCBI): 973
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD79a, also named as B-cell antigen receptor complex-associated protein alpha chain or MB-1 membrane glycoprotein, is a 226 amino acid protein, which contains one ITAM domain and one Ig-like C2-type (immunoglobulin-like) domain. CD79a is expressed in B cell and localizes in the cell membrane. CD79a is required in cooperation with CD79b for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. CD79a is also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells.