Anti-Rat CD86 (24F)

• 

  FC experiment of Wistar Rat splenocytes using 65224-1-Ig

  1X10^6 unstimulated or LPS-stimulated Wistar rat splenocytes were surface stained with 0.5 ug Anti-Rat CD86 (65224-1-Ig, Clone:24F) and FITC anti-mouse IgG1 Antibody at dilution 1:100. Cells were not fixed.

CD86 Monoclonal Antibody for FC

Cat No. 65224-1-Ig

Clone No.24F

Publications(7)

Host / Isotype

Mouse / IgG1, kappa

Reactivity

rat

Applications

IF, FC

Synonyms

CD86 molecule, B7-2, B7 2, 24F

---

Tested Applications

• Positive FC detected in: LPS-stimulated Wistar rat splenocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.5 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 6 publications below
• FC: See 2 publications below

Product Information

65224-1-Ig targets CD86 in IF, FC applications and shows reactivity with rat samples.

• Tested Reactivity: rat
• Cited Reactivity: rat
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  HTLV-1-transformed Lewis-S1 rat T cell line

• Full Name: CD86 molecule
• Calculated Molecular Weight: 36 kDa
• GenBank Accession Number: NM_020081
• Gene Symbol: Cd86
• Gene ID (NCBI): 56822
• RRID: AB_3670278
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: O35531
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD86 (also known as B7.2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
rat	IF	Mater Today Bio; Apoptotic extracellular vesicles derived from MSCs exposed to hypoxic and inflammatory environments slow intervertebral disc degeneration by enhancing cell activity and regulating immunity microenvironment.; Authors - Weiqi Zhang
rat	IF	Curr Issues Mol Biol; IL-37 Ameliorates Chronic Endometritis by Attenuating Epithelial-Mesenchymal Transition and Promoting M2 Macrophage Polarization.; Authors - Zihan Wang
rat	FC	Mater Today Bio; 3D-printed nHA/PA66 porous scaffold: Regulating immune balance and vascularization synergistically promotes bone regeneration.; Authors - Caiping Yan
rat	IF	Mater Today Bio; Acetylated chitosan scaffolds modulate the micro-environment and promote peripheral nerve regeneration through macrophage TLR2/4 pathway activation.; Authors - Yuhui Jiang
rat	IF,FC	Prostaglandins Other Lipid Mediat; Protective role of Yougui Yin in experimental knee osteoarthritis: from the perspective of macrophage polarization; Authors - Zhongqing Wu
rat	IF	Biomaterials; Bioactive prosthetic interface constructed with cascading multi-nanozyme hydrogel to induce M2 macrophage polarization and heal diabetic bone defects.; Authors - Dan Li