FITC Plus Anti-Rat CD86 (24F)

CD86 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

rat

Applications

FC

Conjugate

FITC Plus Fluorescent Dye

CloneNo.

24F

Cat no : FITC-65224

Synonyms

B7 2, Cd86, CD86 molecule

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Validation Data Gallery

• 

  FC experiment of rat splenocytes using FITC-65224

  1X10^6 unstimulated or LPS-treated (5 ug/mL, 3 days) rat splenocytes were surface co-stained with CoraLite® Plus 647 Anti-Rat CD45RA (OX-33) and 0.5 ug FITC Plus Anti-Rat CD86 (FITC-65224, Clone: 24F). Cells were not fixed.

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Tested Applications

• Positive FC detected in: LPS treated rat splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

FITC-65224 targets CD86 in FC applications and shows reactivity with rat samples.

• Tested Reactivity: rat
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: HTLV-1-transformed Lewis-S1 rat T cell line
• Full Name: CD86 molecule
• Calculated Molecular Weight: 36 kDa
• GenBank Accession Number: NM_020081
• Gene Symbol: Cd86
• Gene ID (NCBI): 56822
• Conjugate: FITC Plus Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 495 nm / 524 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD86 (also known as B7.2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response.