Tested Applications
| Positive FC detected in | human PBMCs |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL405-SF98095 targets CD9 in FC applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Type | Rabbit / scFv |
| Class | Recombinant |
| Type | Single-chain variable fragment antibody (scFv) |
| Immunogen |
CatNo: Eg1037 Product name: recombinant human CD9 protein Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS Tag: C-6*HIS Domain: 112-195 aa of NM_001769.4 Sequence: SHKDEVIKEVQEFYKDTYNKLKTKDEPQRETLKAIHYALNCCGLAGGVEQFISDICPKKDVLETFTVKSCPDAIKEVFDNKFHI Predict reactive species |
| Full Name | CD9 molecule |
| Calculated Molecular Weight | 25 kDa |
| GenBank Accession Number | NM_001769.4 |
| Gene Symbol | CD9 |
| Gene ID (NCBI) | 928 |
| RRID | AB_3747210 |
| Conjugate | CoraLite® Plus 405 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 399 nm / 422 nm |
| Excitation Laser | Violet Laser (405 nm) |
| Source | Anti-Human CD9 scFv from clone 240830E6, consisting of VL-GGGGS linker-VH (N- to C-terminus), with a His tag at the C-terminus. |
| Form | Liquid |
| Purification Method | Affinity purification |
| Storage Buffer | 50mM HEPES, 150mM NaCl, 5mM EDTA, 0.1% CHAPS, 10% Sucrose and 0.1% Proclin 300, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. 20ul sizes contain 0.1% BSA. |
Background Information
The cell-surface molecule CD9, a member of the transmembrane-4 superfamily, interacts with the integrin family and other membrane proteins, and is postulated to participate in cell migration and adhesion. Expression of CD9 enhances membrane fusion between muscle cells and promotes viral infection in some cells (PMID:10459022). It is often used as a mesenchymal stem cell marker (PMID:18005405).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 405 CD9 antibody CL405-SF98095 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
